Click image to see more details
Product Info Summary
| SKU: | A01207 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Vinculin/VCL Antibody Picoband®
SKU/Catalog Number
A01207
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Vinculin/VCL Antibody Picoband® catalog # A01207. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Vinculin/VCL Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01207)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Vinculin recombinant protein (Position: E930-Q1134). Human Vinculin shares 87.7% amino acid (aa) sequence identity with both mouse and rat Vinculin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01207 is reactive to VCL in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
124 kDa
Calculated molecular weight
123799 MW
Background of Vinculin
Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01207 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human HepG2 whole cell, human U87 whole cell, rat RH35 whole cell, rat PC-12 whole cell, mouse RAW2647 whole cell, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Vinculin using anti-Vinculin antibody (A01207).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human U87 whole cell lysates,
Lane 3: rat RH35 whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse RAW264.7 whole cell lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vinculin antigen affinity purified polyclonal antibody (Catalog # A01207) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vinculin at approximately 124 kDa. The expected band size for Vinculin is at 124 kDa.
Protein Target Info & Infographic
Gene/Protein Information For VCL (Source: Uniprot.org, NCBI)
Gene Name
VCL
Full Name
Vinculin
Weight
123799 MW
Superfamily
vinculin/alpha-catenin family
Alternative Names
Vinculin;Metavinculin;MV;VCL; VCL CMD1W, CMH15, HEL114, MV, MVCL vinculin vinculin|epididymis luminal protein 114|epididymis secretory sperm binding protein|meta-vinculin|metavinculin|vinculin isoform meta-VCL
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VCL, check out the VCL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VCL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Vinculin/VCL Antibody Picoband® (A01207)
Hello CJ!
A01207 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wang K,Xing ZH,Jiang QW,Yang Y,Huang JR,Yuan ML,Wei MN,Li Y,Wang ST,Liu K,Shi Z.Targeting uPAR by CRISPR/Cas9 System Attenuates Cancer Malignancy and Multidrug Resistance.Front Oncol.2019 Feb 27;9:80.doi:10.3389/fonc.2019.00080.PMID:30873379;PMCID:PMC6400983.
Species: Human
A01207 usage in article: APP:WB, SAMPLE:HCT8/T CELL AND KBV200 CELL, DILUTION:NA
Dong Y,Chen Y,Zhu D,Shi K,Ma C,Zhang W,Rocchi P,Jiang L,Liu X.Self-assembly of amphiphilic phospholipid peptide dendrimer-based nanovectors for effective delivery of siRNA therapeutics in prostate cancer therapy.J Control Release.2020 Jun 10;322:416-425.d
Species: Human,Mouse
A01207 usage in article: APP:WB, SAMPLE:PC-3 CELL, DILUTION:NA
Effects of atorvastatin on porcine aqueous humour outflow and trabecular meshwork cells
Yao Q, An Y, Hou W, Cao YN, Yao MF, Ma NN, Hou L, Zhang H, Liu HJ, Zhang B. Oncotarget. 2017 Nov 30;8(65):109632-109645. doi: 10.18632/oncotarget.22759. eCollection 2017 Dec 12. LRP6 promotes invasion and metastasis of colorectal cancer through cy...
Shen Z, Lu D, Li Q, Zhang Z, Zhu Y. Biomed Res Int. 2015;2015:871202. Doi: 10.1155/2015/871202. Epub 2015 Mar 8. Synthesis And Characterization Of Biodegradable Polyurethane For Hypopharyngeal Tissue Engineering.
Su F,, Liu Ss,, Ma Jl, Wang Ds, E Ll, Liu Hc. Stem Cell Res Ther. 2015 Mar 12;6:22. Doi: 10.1186/S13287-015-0023-3. Enhancement Of Periodontal Tissue Regeneration By Transplantation Of Osteoprotegerin-Engineered Periodontal Ligament Stem Cells.
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Vinculin/VCL Antibody Picoband®
Question
We need to test anti-Vinculin/VCL antibody A01207 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-Vinculin/VCL antibody A01207 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
L. Patel
Verified customer
Asked: 2020-04-29
Answer
The products we sell, including anti-Vinculin/VCL antibody A01207, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-29
Question
We are currently using anti-Vinculin/VCL antibody A01207 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
The anti-Vinculin/VCL antibody (A01207) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-13
Question
Will anti-Vinculin/VCL antibody A01207 work for WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
According to the expression profile of cervix carcinoma erythroleukemia, VCL is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-Vinculin/VCL antibody A01207 will work for WB with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2020-03-18
Question
Is a blocking peptide available for product anti-Vinculin/VCL antibody (A01207)?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
We do provide the blocking peptide for product anti-Vinculin/VCL antibody (A01207). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-27
Question
Can you help my question with product A01207, anti-Vinculin/VCL antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01207 anti-Vinculin/VCL antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-14
Question
Is there a BSA free version of anti-Vinculin/VCL antibody A01207 available?
Verified Customer
Verified customer
Asked: 2019-04-25
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Vinculin/VCL antibody A01207 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-04-25
Question
Is this A01207 anti-Vinculin/VCL antibody reactive to the isotypes of VCL?
Verified Customer
Verified customer
Asked: 2018-12-28
Answer
The immunogen of A01207 anti-Vinculin/VCL antibody is E.coli-derived human Vinculin recombinant protein (Position: E930-Q1134). Human Vinculin shares 87.7% amino acid (aa) sequence identity with both mouse and rat Vinculin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-12-28
Question
Would A01207 anti-Vinculin/VCL antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-04-25
Answer
It shows on the product datasheet, A01207 anti-Vinculin/VCL antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-04-25
Question
Please see the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Vinculin/VCL antibody A01207. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-01-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-10
Question
I was wanting to use your anti-Vinculin/VCL antibody for WB for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2017-11-30
Answer
You can see on the product datasheet, A01207 anti-Vinculin/VCL antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-11-30
Question
I see that the anti-Vinculin/VCL antibody A01207 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-11-30
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-11-30
Question
We are interested in using your anti-Vinculin/VCL antibody for cell adhesion studies. Has this antibody been tested with western blotting on a549 whole cell lysates? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-08-01
Answer
We appreciate your inquiry. This A01207 anti-Vinculin/VCL antibody is tested on a549 whole cell lysates. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-08-01
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Vinculin/VCL antibody A01207. Let me know if you need anything else.
G. Miller
Verified customer
Asked: 2017-01-16
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-01-16
Question
My boss were content with the WB result of your anti-Vinculin/VCL antibody. However we have seen positive staining in prostate cell membrane using this antibody. Is that expected? Could you tell me where is VCL supposed to be expressed?
S. Johnson
Verified customer
Asked: 2016-08-26
Answer
Based on literature, prostate does express VCL. Generally VCL expresses in cell membrane. Regarding which tissues have VCL expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Endothelial cell, Pubmed ID: 2116004
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 12665801
Prostate, Pubmed ID: 15489334
Retina, Pubmed ID: 17974005
Uterus, Pubmed ID: 1339348
Boster Scientific Support
Answered: 2016-08-26
Question
We have seen staining in human prostate. Do you have any suggestions? Is anti-Vinculin/VCL antibody supposed to stain prostate positively?
P. Carter
Verified customer
Asked: 2014-01-02
Answer
From literature prostate does express VCL. From Uniprot.org, VCL is expressed in myometrium, endothelial cell, retina, prostate, fetal brain cortex, uterus, platelet, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have VCL expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Endothelial cell, Pubmed ID: 2116004
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 12665801
Prostate, Pubmed ID: 15489334
Retina, Pubmed ID: 17974005
Uterus, Pubmed ID: 1339348
Boster Scientific Support
Answered: 2014-01-02
