Anti-VEGF (Vascular Endothelial Growth Factor) VEGFA Monoclonal Antibody

Product Name

Anti-VEGF (Vascular Endothelial Growth Factor) VEGFA Monoclonal Antibody

View all VEGFA Antibodies

SKU/Catalog Number

M00045

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-VEGF (Vascular Endothelial Growth Factor) VEGFA Monoclonal Antibody (Catalog # M00045). Tested in IF, IHC applications. This antibody reacts with Human, Mouse, Rat, Rabbit, Dog.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-VEGF (Vascular Endothelial Growth Factor) VEGFA Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00045)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM225

Isotype

IgG1, kappa

Immunogen

Human VEGF189 recombinant protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00045 is reactive to VEGFA in Dog, Human, Mouse, Rabbit, Rat

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Calculated molecular weight

27042 MW

Background of VEGFA

This monoclonal antibody recognizes proteins of 19-22kDa (reducing) and 38kDa-44kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00045 is guaranteed for IF, IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (4-8ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA buffer, pH 8.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For VEGFA (Source: Uniprot.org, NCBI)

Alternative Names

Vascular endothelial growth factor A;VEGF-A;Vascular permeability factor;VPF;VEGFA;VEGF; VEGFA MVCD1, VEGF, VPF vascular endothelial growth factor A vascular endothelial growth factor A|vascular endothelial growth factor A121|vascular endothelial growth factor A165|vascular permeability factor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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