Click image to see more details
Product Info Summary
| SKU: | A00045-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-VEGF/Vegfa/VEGF164 Antibody Picoband®
SKU/Catalog Number
A00045-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-VEGF/Vegfa/VEGF164 Antibody Picoband® catalog # A00045-1. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VEGF/Vegfa/VEGF164 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00045-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse VEGF/VEGF164 recombinant protein (Position: A27-R190).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00045-1 is reactive to Vegfa in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
27 kDa, 40 kDa
Calculated molecular weight
25283 MW
Background of VEGFA
VEGF, a homodimeric glycoprotein of relative molecular mass 45,000, is the only mitogen that specifically acts on endothelial cells. It may be a major regulator of tumor angiogenesis in vivo. It is, however, structurally related to platelet-derived growth factor. VEGF shares homology with the PDGF A chain and B chain, including conservation of all 8 cysteines found in PDGFA and PDGFB. VEGF gene contains 8 exons. And VEGF induces remodeling and enhances TH2-mediated sensitization and inflammation in the lung. This gene can also regulate haematopoietic stem cell survival by an internal autocrine loop mechanism. What’s more, it also stimulates neurogenesis in vitro and in vivo.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00045-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: mouse thymus tissue, mouse HEPA1-6 whole cell, rat C6 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of VEGF/VEGF164 using anti-VEGF/VEGF164 antibody (A00045-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse thymus tissue lysates,
Lane 2: mouse HEPA1-6 whole cell lysates,
Lane 3: rat C6 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF/VEGF164 antigen affinity purified polyclonal antibody (Catalog # A00045-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGF/VEGF164 at approximately 27KD, 40KD. The expected band size for VEGF/VEGF164 is at 25KD.
Protein Target Info & Infographic
Gene/Protein Information For Vegfa (Source: Uniprot.org, NCBI)
Gene Name
Vegfa
Full Name
Vascular endothelial growth factor A
Weight
25283 MW
Superfamily
PDGF/VEGF growth factor family
Alternative Names
Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF; Vegfa; Vegf VEGFA MVCD1, VEGF, VPF vascular endothelial growth factor A vascular endothelial growth factor A|vascular endothelial growth factor A121|vascular endothelial growth factor A165|vascular permeability factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Vegfa, check out the Vegfa Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Vegfa: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VEGF/Vegfa/VEGF164 Antibody Picoband® (A00045-1)
Hello CJ!
A00045-1 has been cited in 50 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The role of bone morphogenetic protein 4 in corneal injury repair
Zhou D,Huang X,Xie Y,Deng Z,Guo J,Huang H.Astrocytes-derived VEGF exacerbates the microvascular damage of late delayed RBI.Neuroscience.2019 Jun 1;408:14-21.doi:10.1016/j.neuroscience.2019.03.039.Epub 2019 Mar 22.PMID:30910640.
Species: Mouse
A00045-1 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:1:300
Deer thymosin beta 10 functions as a novel factor for angiogenesis and chondrogenesis during antler growth and regeneration
Inhibition of angiogenesis by arsenic trioxide via TSP-1?TGF-?1-CTGF?VEGF functional module in rheumatoid arthritis
Epidermal growth factor receptor transactivation is involved in the induction of human hepatoma SMMC7721 cell proliferation by insufficient radiofrequency ablation
miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression
IL-17 Promotes Angiogenic Factors IL-6, IL-8, and Vegf Production via Stat1 in Lung Adenocarcinoma
Vitamin K2 Ameliorates Damage of Blood Vessels by Glucocorticoid: a Potential Mechanism for Its Protective Effects in Glucocorticoid-induced Osteonecrosis of the Femoral Head in a Rat Model
Proper autophagy is indispensable for angiogenesis during chick embryo development
VEGFR-2 conformational switch in response to ligand binding
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-VEGF/Vegfa/VEGF164 Antibody Picoband®
Question
I see that the anti-VEGF/Vegfa/VEGF164 antibody A00045-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-24
Question
Is a blocking peptide available for product anti-VEGF/Vegfa/VEGF164 antibody (A00045-1)?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
We do provide the blocking peptide for product anti-VEGF/Vegfa/VEGF164 antibody (A00045-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-13
Question
Do you have a BSA free version of anti-VEGF/Vegfa/VEGF164 antibody A00045-1 available?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-VEGF/Vegfa/VEGF164 antibody A00045-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-29
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-VEGF/Vegfa/VEGF164 antibody A00045-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-13
Question
My question regarding product A00045-1, anti-VEGF/Vegfa/VEGF164 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Singh
Verified customer
Asked: 2019-11-18
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00045-1 anti-VEGF/Vegfa/VEGF164 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-18
Question
I have attached the WB image, lot number and protocol we used for lung using anti-VEGF/Vegfa/VEGF164 antibody A00045-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-22
Question
We want to test anti-VEGF/Vegfa/VEGF164 antibody A00045-1 on mouse lung for research purposes, then I may be interested in using anti-VEGF/Vegfa/VEGF164 antibody A00045-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
The products we sell, including anti-VEGF/Vegfa/VEGF164 antibody A00045-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-24
Question
Is this A00045-1 anti-VEGF/Vegfa/VEGF164 antibody reactive to the isotypes of VEGFA?
T. Johnson
Verified customer
Asked: 2019-08-19
Answer
The immunogen of A00045-1 anti-VEGF/Vegfa/VEGF164 antibody is E. coli-derived mouse VEGF/VEGF164 recombinant protein (Position: A27-R190). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-19
Question
We have observed staining in mouse renal glomerulus. What should we do? Is anti-VEGF/Vegfa/VEGF164 antibody supposed to stain renal glomerulus positively?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
Based on literature renal glomerulus does express VEGFA. Based on Uniprot.org, VEGFA is expressed in left lobe of thyroid gland, kidney, mammary gland, renal glomerulus, hemangioendothelioma, heart, lung, retina, among other tissues. Regarding which tissues have VEGFA expression, here are a few articles citing expression in various tissues:
Heart, Pubmed ID: 19054851
Kidney, Pubmed ID: 9878851, 12124351
Lung, Pubmed ID: 15489334
Mammary gland, Pubmed ID: 9450968
Renal glomerulus, Pubmed ID: 10464055
Retina, Pubmed ID: 10067980, 11563986
Boster Scientific Support
Answered: 2019-05-24
Question
We are currently using anti-VEGF/Vegfa/VEGF164 antibody A00045-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
The anti-VEGF/Vegfa/VEGF164 antibody (A00045-1) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-14
Question
Will A00045-1 anti-VEGF/Vegfa/VEGF164 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Edwards
Verified customer
Asked: 2019-02-15
Answer
You can see on the product datasheet, A00045-1 anti-VEGF/Vegfa/VEGF164 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-02-15
Question
We ordered your anti-VEGF/Vegfa/VEGF164 antibody for WB on lung a few years ago. I am using mouse, and We are going to use the antibody for ELISA next. My question regards examining lung as well as heart in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?
Verified Customer
Verified customer
Asked: 2018-12-10
Answer
I looked at the website and datasheets of our anti-VEGF/Vegfa/VEGF164 antibody and it appears that A00045-1 has been tested on mouse in both WB and ELISA. Thus A00045-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-12-10
Question
My team were well pleased with the WB result of your anti-VEGF/Vegfa/VEGF164 antibody. However we have seen positive staining in left lobe of thyroid gland secreted using this antibody. Is that expected? Could you tell me where is VEGFA supposed to be expressed?
A. Wu
Verified customer
Asked: 2018-07-05
Answer
From literature, left lobe of thyroid gland does express VEGFA. Generally VEGFA expresses in secreted. Regarding which tissues have VEGFA expression, here are a few articles citing expression in various tissues:
Heart, Pubmed ID: 19054851
Kidney, Pubmed ID: 9878851, 12124351
Lung, Pubmed ID: 15489334
Mammary gland, Pubmed ID: 9450968
Renal glomerulus, Pubmed ID: 10464055
Retina, Pubmed ID: 10067980, 11563986
Boster Scientific Support
Answered: 2018-07-05
Question
I was wanting to use your anti-VEGF/Vegfa/VEGF164 antibody for WB for mouse lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse lung identification?
Verified Customer
Verified customer
Asked: 2018-05-30
Answer
As indicated on the product datasheet, A00045-1 anti-VEGF/Vegfa/VEGF164 antibody has been tested for ELISA, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-30
Question
Would anti-VEGF/Vegfa/VEGF164 antibody A00045-1 work for WB with lung?
Verified Customer
Verified customer
Asked: 2017-08-16
Answer
According to the expression profile of lung, VEGFA is highly expressed in lung. So, it is likely that anti-VEGF/Vegfa/VEGF164 antibody A00045-1 will work for WB with lung.
Boster Scientific Support
Answered: 2017-08-16
Question
you antibody using your anti-VEGF/Vegfa/VEGF164 antibody for positive regulation of dna biosynthetic process studies. Has this antibody been tested with western blotting on rat c6 whole cell lysates? We would like to see some validation images before ordering.
P. Thomas
Verified customer
Asked: 2014-10-08
Answer
Thank you for your inquiry. This A00045-1 anti-VEGF/Vegfa/VEGF164 antibody is tested on mouse thymus tissue, rat c6 whole cell lysates. It is guaranteed to work for ELISA, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2014-10-08
