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Product Info Summary
| SKU: | M07816 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, ICC, WB |
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Product info
Product Name
Anti-USP13 Monoclonal Antibody
SKU/Catalog Number
M07816
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-USP13 Monoclonal Antibody catalog # M07816. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-USP13 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07816)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADDG-21
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human USP13 Mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M07816 is reactive to USP13 in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
83 kDa
Calculated molecular weight
25279 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M07816 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
FC 1:100
Positive Control
WB: HepG2 cell
Validation Images & Assay Conditions
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Western blot analysis of USP13 expression in HepG2 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For USP13 (Source: Uniprot.org, NCBI)
Gene Name
USP13
Full Name
Ubiquitin carboxyl-terminal hydrolase 13
Weight
25279 MW
Superfamily
peptidase C19 family
Alternative Names
Ubiquitin-like-conjugating enzyme ATG10;6.3.2.-;Autophagy-related protein 10;APG10-like;ATG10;APG10L;PP12616; USP13 ISOT3, IsoT-3 ubiquitin specific peptidase 13 ubiquitin carboxyl-terminal hydrolase 13|deubiquitinating enzyme 13|ubiquitin specific peptidase 13 (isopeptidase T-3)|ubiquitin specific protease 13 (isopeptidase T-3)|ubiquitin thioesterase 13|ubiquitin thiolesterase 13|ubiquitin-specific-processing protease 13
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on USP13, check out the USP13 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for USP13: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-USP13 Monoclonal Antibody (M07816)
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No publications found for M07816
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-USP13 Monoclonal Antibody
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for uterus using anti-USP13 Monoclonal antibody M07816. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-13
Question
Does anti-USP13 Monoclonal antibody M07816 work on monkey IF with testis tongue?
Verified Customer
Verified customer
Asked: 2020-04-10
Answer
Our lab technicians have not tested anti-USP13 Monoclonal antibody M07816 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-USP13 Monoclonal antibody M07816 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey testis tongue in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-10
Question
Is a blocking peptide available for product anti-USP13 Monoclonal antibody (M07816)?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
We do provide the blocking peptide for product anti-USP13 Monoclonal antibody (M07816). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-09
Question
My question regarding product M07816, anti-USP13 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M07816 anti-USP13 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-13
Question
See below the WB image, lot number and protocol we used for uterus using anti-USP13 Monoclonal antibody M07816. Please let me know if you require anything else.
B. Jones
Verified customer
Asked: 2014-01-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-01-30
Question
We are currently using anti-USP13 Monoclonal antibody M07816 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on primate tissues as well?
R. Jha
Verified customer
Asked: 2013-05-16
Answer
The anti-USP13 Monoclonal antibody (M07816) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-05-16
