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Product Info Summary
| SKU: | EK0848 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants and cell lysates. |
Product info
Product Name
Human TrkB ELISA Kit PicoKine®
SKU/Catalog Number
EK0848
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human TrkB ELISA Kit PicoKine® (96 Tests). Quantitate Human NTRK2 in cell culture supernatants and cell lysates. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human TrkB ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0848)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: C32-H430
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0848 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0848 is reactive to NTRK2 in Human samples
Validated Sample Types
cell culture supernatants and cell lysates.
Application Guarantee
EK0848 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TrkB
TrkB receptor also known as TrkB tyrosine kinase or BDNF/NT-3 growth factors receptor or neurotrophic tyrosine kinase, receptor, type 2 is a protein that in humans is encoded by the NTRK2 gene.[1] TrkB is the high affinity catalytic receptor for several "neurotrophins", which are small protein growth factors that induce the survival and differentiation of distinct cell populations. The TrkB receptor is part of the large family of receptor tyrosine kinases. A "tyrosine kinase" is an enzyme which is capable of adding a phosphate group to certain tyrosines on target proteins, or "substrates".Soppet et al. [2] demonstrated that the gp145 gene product of the TRKB gene is rapidly phosphorylated on tyrosine residues upon exposure to BDNF and NTF3.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0848-CAP | Anti-Human NTRK2 Pre-coated 96-well strip microplate | 1 |
| EK0848-ST | Human NTRK2 Standard | 2 vials, 10 ng/tube |
| EK0848-DA | Human NTRK2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.009 | 0.084 | 0.149 | 0.269 | 0.459 | 0.818 | 1.530 | 2.012 |
Data Example Images
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Human TrkB PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human TrkB ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 267 | 1279 | 4273 | 292 | 1238 | 3991 |
| Standard deviation | 18.69 | 88.85 | 260.65 | 26.99 | 91.61 | 259.41 |
| CV (%) | 7% | 6.9% | 6.1% | 8.8% | 7.4% | 6.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of TrkB in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 267 | 270 | 268 | 254 | 264 | 6.29 | 2.3% |
| Sample 2 | 1279 | 1382 | 1154 | 1370 | 1296 | 91.27 | 7% |
| Sample 3 | 4273 | 4945 | 4447 | 4961 | 4656 | 302.86 | 6.5% |
Protein Target Info & Infographic
Gene/Protein Information For NTRK2 (Source: Uniprot.Org, NCBI)
Gene Name
NTRK2
Full Name
BDNF/NT-3 growth factors receptor
Weight
91999 MW
Superfamily
protein kinase superfamily
Alternative Names
BDNF/NT-3 growth factors receptor;2.7.10.1;GP145-TrkB;Trk-B;Neurotrophic tyrosine kinase receptor type 2;TrkB tyrosine kinase;Tropomyosin-related kinase B;NTRK2;TRKB; NTRK2 DEE58, EIEE58, GP145-TrkB, OBHD, TRKB, trk-B neurotrophic receptor tyrosine kinase 2 BDNF/NT-3 growth factors receptor|BDNF-tropomyosine receptor kinase B|neurotrophic tyrosine kinase receptor type 2|tropomyosin-related kinase B|tyrosine kinase receptor B
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on NTRK2, check out the NTRK2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for NTRK2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human TrkB ELISA Kit PicoKine® (EK0848)
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Customer Q&As
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5 Customer Q&As for Human TrkB ELISA Kit PicoKine®
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring TrkB using the EK0848 Human TrkB Picokine® ELISA Kit?
H. Phillips
Verified customer
Asked: 2019-10-16
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2019-10-16
Question
Q: how to thaw whole blood sample for TrkB ELISA after freezing?
Y. Thompson
Verified customer
Asked: 2019-07-13
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TrkB for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-07-13
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Z. Perez
Verified customer
Asked: 2019-03-19
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-03-19
Question
Q: if the enzyme conjugated TrkB antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TrkB antigen?
M. Patel
Verified customer
Asked: 2017-04-13
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2017-04-13
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2014-07-26
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2014-07-26
