Click image to see more details
Product Info Summary
| SKU: | A01388-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-TrkB (Ab-705) NTRK2 Antibody
SKU/Catalog Number
A01388-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-TrkB (Ab-705) NTRK2 Antibody (Catalog # A01388-1). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TrkB (Ab-705) NTRK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01388-1)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.703~707 (T-D-Y -Y-R) derived from Human TrkB.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01388-1 is reactive to NTRK2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
69217 MW
Background of TrkB
Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.
Woronowicz A, et al. Glycobiology. 2007 Jan;17(1):10-24. Mojsilovic-Petrovic J, et al. J Neurosci. 2006 Sep 6;26(36):9250-63. Lewis MA, et al. Mol Pharmacol. 2006 Apr;69(4):1396-404. Cai D, et al. Physiol Genomics. 2006 Feb 14;24(3):191-7.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01388-1 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 145kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NTRK2 using anti-NTRK2 antibody (A01388-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NTRK2 antigen affinity purified polyclonal antibody (Catalog # A01388-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for NTRK2.
Click image to see more details
Immunofluorescence staining of methanol-fixed NCI-h446 cells showing cytoplasmic staining using TrkB (Ab-705) Antibody #A01388-1.
Protein Target Info & Infographic
Gene/Protein Information For NTRK2 (Source: Uniprot.org, NCBI)
Gene Name
NTRK2
Full Name
BDNF/NT-3 growth factors receptor
Weight
69217 MW
Superfamily
protein kinase superfamily
Alternative Names
Sphingosine kinase 2;SK 2;SPK 2;2.7.1.91;SPHK2; NTRK2 DEE58, EIEE58, GP145-TrkB, OBHD, TRKB, trk-B neurotrophic receptor tyrosine kinase 2 BDNF/NT-3 growth factors receptor|BDNF-tropomyosine receptor kinase B|neurotrophic tyrosine kinase receptor type 2|tropomyosin-related kinase B|tyrosine kinase receptor B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NTRK2, check out the NTRK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NTRK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TrkB (Ab-705) NTRK2 Antibody (A01388-1)
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-TrkB (Ab-705) NTRK2 Antibody
Question
Is a blocking peptide available for product anti-TrkB (Ab-705) antibody (A01388-1)?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
We do provide the blocking peptide for product anti-TrkB (Ab-705) antibody (A01388-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-30
Question
I am looking for to test anti-TrkB (Ab-705) antibody A01388-1 on mouse brain for research purposes, then I may be interested in using anti-TrkB (Ab-705) antibody A01388-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-19
Answer
The products we sell, including anti-TrkB (Ab-705) antibody A01388-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-19
Question
Will A01388-1 anti-TrkB (Ab-705) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
You can see on the product datasheet, A01388-1 anti-TrkB (Ab-705) antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-16
Question
My question regarding product A01388-1, anti-TrkB (Ab-705) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01388-1 anti-TrkB (Ab-705) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-24
Question
Is this A01388-1 anti-TrkB (Ab-705) antibody reactive to the isotypes of NTRK2?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
The immunogen of A01388-1 anti-TrkB (Ab-705) antibody is Peptide sequence around aa.703~707 (T-D-Y -Y-R) derived from Human TrkB. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-18
Question
We have seen staining in mouse amygdala. What should we do? Is anti-TrkB (Ab-705) antibody supposed to stain amygdala positively?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
From what I have seen in literature amygdala does express NTRK2. From what I have seen in Uniprot.org, NTRK2 is expressed in hypothalamus, hippocampus, brain, amygdala, plasma, among other tissues. Regarding which tissues have NTRK2 expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 14702039
Brain, Pubmed ID: 7823156, 15164053, 15489334
Hippocampus, Pubmed ID: 7789988, 7936202
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2019-12-16
Question
I see that the anti-TrkB (Ab-705) antibody A01388-1 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-16
Question
Will anti-TrkB (Ab-705) antibody A01388-1 work for IF with brain?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
According to the expression profile of brain, NTRK2 is highly expressed in brain. So, it is likely that anti-TrkB (Ab-705) antibody A01388-1 will work for IF with brain.
Boster Scientific Support
Answered: 2019-12-16
Question
We have tried in the past anti-TrkB (Ab-705) antibody for WB on hypothalamus a few months ago. I am using rat, and We intend to use the antibody for IF next. you antibody examining hypothalamus as well as brain in our next experiment. Could give a recommendation on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
I have checked the website and datasheets of our anti-TrkB (Ab-705) antibody and it appears that A01388-1 has been tested on rat in both WB and IF. Thus A01388-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-09-19
Question
Is there a BSA free version of anti-TrkB (Ab-705) antibody A01388-1 available?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TrkB (Ab-705) antibody A01388-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-26
Question
My team were satisfied with the WB result of your anti-TrkB (Ab-705) antibody. However we have observed positive staining in hippocampus cell membrane using this antibody. Is that expected? Could you tell me where is NTRK2 supposed to be expressed?
T. Lewis
Verified customer
Asked: 2018-08-15
Answer
Based on literature, hippocampus does express NTRK2. Generally NTRK2 expresses in cell membrane. Regarding which tissues have NTRK2 expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 14702039
Brain, Pubmed ID: 7823156, 15164053, 15489334
Hippocampus, Pubmed ID: 7789988, 7936202
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2018-08-15
Question
Here is the WB image, lot number and protocol we used for brain using anti-TrkB (Ab-705) antibody A01388-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-05-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-02
Question
We are currently using anti-TrkB (Ab-705) antibody A01388-1 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
D. Patel
Verified customer
Asked: 2017-07-05
Answer
The anti-TrkB (Ab-705) antibody (A01388-1) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-05
Question
I was wanting to use your anti-TrkB (Ab-705) antibody for IF for mouse brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse brain identification?
Verified Customer
Verified customer
Asked: 2017-06-19
Answer
You can see on the product datasheet, A01388-1 anti-TrkB (Ab-705) antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-19
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-TrkB (Ab-705) antibody A01388-1. Let me know if you need anything else.
D. Walker
Verified customer
Asked: 2015-02-17
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-02-17
