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Product Info Summary
| SKU: | M01970 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Ubiquitin D UBD Rabbit Monoclonal Antibody
SKU/Catalog Number
M01970
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Ubiquitin D UBD Rabbit Monoclonal Antibody catalog # M01970. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ubiquitin D UBD Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01970)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HID-21
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Ubiquitin D
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01970 is reactive to UBD in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
139 kDa
Calculated molecular weight
18473 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01970 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of Ubiquitin D expression in HepG2 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For UBD (Source: Uniprot.org, NCBI)
Gene Name
UBD
Full Name
Ubiquitin D
Weight
18473 MW
Alternative Names
Ubiquitin D;Diubiquitin;Ubiquitin-like protein FAT10;UBD;FAT10; UBD FAT10, GABBR1-3, UBD ubiquitin D ubiquitin D|diubiquitin|ubiquitin-like protein FAT10
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UBD, check out the UBD Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UBD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ubiquitin D UBD Rabbit Monoclonal Antibody (M01970)
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No publications found for M01970
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Customer Q&As
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5 Customer Q&As for Anti-Ubiquitin D UBD Rabbit Monoclonal Antibody
Question
Does anti-Ubiquitin D Rabbit Monoclonal antibody M01970 work for WB with urinary bladder?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
According to the expression profile of urinary bladder, UBD is highly expressed in urinary bladder. So, it is likely that anti-Ubiquitin D Rabbit Monoclonal antibody M01970 will work for WB with urinary bladder.
Boster Scientific Support
Answered: 2020-01-15
Question
I see that the anti-Ubiquitin D Rabbit Monoclonal antibody M01970 works with WB, what is the protocol used to produce the result images on the product page?
C. Jackson
Verified customer
Asked: 2019-07-29
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-29
Question
Would M01970 anti-Ubiquitin D Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
It shows on the product datasheet, M01970 anti-Ubiquitin D Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-27
Question
Would anti-Ubiquitin D Rabbit Monoclonal antibody M01970 work on zebrafish IF with urinary bladder?
Verified Customer
Verified customer
Asked: 2019-03-29
Answer
Our lab technicians have not validated anti-Ubiquitin D Rabbit Monoclonal antibody M01970 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Ubiquitin D Rabbit Monoclonal antibody M01970 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish urinary bladder in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-29
Question
I was wanting to use your anti-Ubiquitin D Rabbit Monoclonal antibody for WB for mouse urinary bladder on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse urinary bladder identification?
Verified Customer
Verified customer
Asked: 2018-08-20
Answer
You can see on the product datasheet, M01970 anti-Ubiquitin D Rabbit Monoclonal antibody has been tested for IF, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse urinary bladder in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-20
