Anti-Diubiquitin/UBD Antibody Picoband®

Product Name

Anti-Diubiquitin/UBD Antibody Picoband®

View all FAT10 Antibodies

SKU/Catalog Number

PA2222

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Diubiquitin/UBD Antibody catalog # PA2222. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Diubiquitin/UBD Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2222)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Diubiquitin, different from the related mouse and rat sequences by five amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2222 is reactive to UBD in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

91 kDa

Calculated molecular weight

18473 MW

Background of FAT10

Ubiquitin D, also called UBD or FAT10 is a protein that in humans is encoded by the UBD gene. This gene is mapped to 6p22.1. Ubiquitin-like protein modifier can be covalently attached to target protein and subsequently leads to their degradation by the 26S proteasome in a NUB1L-dependent manner. This gene may be involved in dendritic cell (DC) maturation, the process by which immature dendritic cells differentiate into fully competent antigen-presenting cells that initiate T-cell responses. It may be involved in the formation of aggresomes when proteasome is saturated or impaired. This gene mediates apoptosis in a caspase-dependent manner, especially in renal epithelium and tubular cells during renal diseases such as polycystic kidney disease and Human immunodeficiency virus (HIV)-associated nephropathy (HIVAN). It probably functions as a survival factor.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA2222 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Positive Control

WB: HELA Cell, SKOV Cell, MCF-7 Cell, A549 Cell, SMMC Cell
IHC: Human Intestinal Cancer Tissue, Human Tonsil Tissue

Validation Images & Assay Conditions

Gene/Protein Information For UBD (Source: Uniprot.org, NCBI)

Alternative Names

Ubiquitin D;Diubiquitin;Ubiquitin-like protein FAT10;UBD;FAT10; UBD FAT10, GABBR1-3, UBD ubiquitin D ubiquitin D|diubiquitin|ubiquitin-like protein FAT10

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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