Product Info Summary
SKU: | M04387 |
---|---|
Size: | 100uL |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-eIF-2 alpha (pS51) EIF2S1 Rabbit Monoclonal Antibody, Clone#RM298
View all eIF2 alpha/EIF2S1 Antibodies
SKU/Catalog Number
M04387
Size
100uL
Form
Liquid
Description
Boster Bio Anti-eIF-2 alpha (pS51) EIF2S1 Rabbit Monoclonal Antibody, Clone#RM298 (Catalog # M04387). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-eIF-2 alpha (pS51) EIF2S1 Rabbit Monoclonal Antibody, Clone#RM298 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04387)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM298
Isotype
Rabbit IgG
Immunogen
A phospho-peptide corresponding to human Phospho- eIF-2a (Ser51)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Reacts to human eIF-2a (eukaryotic translation initiation factor 2 subunit alpha) only when phosphorylated at Ser51. No cross-reactivity to eIF-2a without phosphorylation at Ser51. May also react to mouse or rat Phospho-eIF-2a (Ser51).
Reactive Species
M04387 is reactive to EIF2S1 in Human
Reconstitution
Calculated molecular weight
36112 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04387 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:100-1:200 dilution
WB: 1:100-1:200 dilution.
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western Blotting result
Western Blot of HeLa cell lysate nontreated (-) or treated (+) with Calyculin A (CalA), using anti-phospho-eIF-2 (Ser51) rabbit monoclonal antibody (clone RM298) at a 1:200 dilution.
Click image to see more details
Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue section using anti-phospho-eIF-2 (Ser51) rabbit monoclonal antibody (clone RM298) at a 1:200 dilution.
Click image to see more details
Figure 3. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human thyroid cancer tissue section using anti-phospho-eIF-2 (Ser51) rabbit monoclonal antibody (clone RM298) at a 1:200 dilution.
Protein Target Info & Infographic
Gene/Protein Information For EIF2S1 (Source: Uniprot.org, NCBI)
Gene Name
EIF2S1
Full Name
Eukaryotic translation initiation factor 2 subunit 1
Weight
36112 MW
Superfamily
eIF-2-alpha family
Alternative Names
Eukaryotic translation initiation factor 2 subunit 1;Eukaryotic translation initiation factor 2 subunit alpha;eIF-2-alpha;eIF-2A;eIF-2alpha;EIF2S1;EIF2A; EIF2S1 EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A eukaryotic translation initiation factor 2 subunit alpha eukaryotic translation initiation factor 2 subunit 1|eIF-2-alpha|eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EIF2S1, check out the EIF2S1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EIF2S1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-eIF-2 alpha (pS51) EIF2S1 Rabbit Monoclonal Antibody, Clone#RM298 (M04387)
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