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Product Info Summary
| SKU: | A00326-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Tyrosinase/TYR Antibody Picoband®
View all Tyrosinase Antibodies
SKU/Catalog Number
A00326-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Tyrosinase/TYR Antibody Picoband® catalog # A00326-3. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Tyrosinase/TYR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00326-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Tyrosinase/TYR recombinant protein (Position: E130-K529).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00326-3 is reactive to TYR in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
60-65 kDa
Calculated molecular weight
29041 MW
Background of Tyrosinase
The enzyme encoded by this gene catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The enzyme has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this gene result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation. The human genome contains a pseudogene similar to the 3' half of this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00326-3 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human A375 whole cell, human A431 whole cell, human Hacat whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Tyrosinase/TYR using anti-Tyrosinase/TYR antibody (A00326-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A375 whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human Hacat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tyrosinase/TYR antigen affinity purified polyclonal antibody (Catalog # A00326-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tyrosinase/TYR at approximately 60-65 kDa. The expected band size for Tyrosinase/TYR is at 60 kDa.
Protein Target Info & Infographic
Gene/Protein Information For TYR (Source: Uniprot.org, NCBI)
Gene Name
TYR
Full Name
Tyrosinase
Weight
29041 MW
Superfamily
tyrosinase family
Alternative Names
Bone morphogenetic protein receptor type-2; BMP type-2 receptor; BMPR-2; Bone morphogenetic protein receptor type II; BMP type II receptor; BMPR-II; BMPR2; PPH1 TYR ATN, CMM8, OCA1, OCA1A, OCAIA, SHEP3 tyrosinase tyrosinase|LB24-AB|SK29-AB|monophenol monooxygenase|oculocutaneous albinism IA|tumor rejection AB
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TYR, check out the TYR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TYR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Tyrosinase/TYR Antibody Picoband® (A00326-3)
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