Product Info Summary
SKU: | M00326 |
---|---|
Size: | 100ug/vial |
Reactive Species: | Human |
Host: | Mouse |
Application: | Flow Cytometry, IF, IHC |
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Product info
Product Name
Anti-Tyrosinase (Melanoma Marker) Monoclonal Antibody
View all Tyrosinase Antibodies
SKU/Catalog Number
M00326
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Tyrosinase (Melanoma Marker) Monoclonal Antibody (Catalog # M00326). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Tyrosinase (Melanoma Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00326)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: T311 + OCA1/812
Isotype
IgG2a, kappa
Immunogen
Recombinant tyrosinase protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00326 is reactive to TYR in Human
Reconstitution
Reconstitute with 1ml water (15 minutes at room temperature).
Calculated molecular weight
60393 MW
Background of Tyrosinase
Recognizes a cluster of proteins between 70-80kDa, identified as tyrosinase. Occasionally a minor band at 55kDa is also detected. This monoclonal antibody shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway; the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with This monoclonal antibody shows tyrosinase in melanotic as well as amelanotic variants. This monoclonal antibody is a useful marker for melanocytes and melanomas.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00326 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human melanoma stained with Anti-Tyrosinase Mouse Monoclonal Antibody (T311 + OCA1/812).
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Formalin-fixed, paraffin-embedded human melanoma stained with Anti-Tyrosinase Mouse Monoclonal Antibody (T311 + OCA1/812).
Protein Target Info & Infographic
Gene/Protein Information For TYR (Source: Uniprot.org, NCBI)
Gene Name
TYR
Full Name
Tyrosinase
Weight
60393 MW
Superfamily
tyrosinase family
Alternative Names
Tyrosinase;1.14.18.1;LB24-AB;Monophenol monooxygenase;SK29-AB;Tumor rejection antigen AB;TYR; TYR ATN, CMM8, OCA1, OCA1A, OCAIA, SHEP3 tyrosinase tyrosinase|LB24-AB|SK29-AB|monophenol monooxygenase|oculocutaneous albinism IA|tumor rejection AB
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TYR, check out the TYR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TYR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Tyrosinase (Melanoma Marker) Monoclonal Antibody (M00326)
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