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Product Info Summary
| SKU: | M06313 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Monkey, Mouse, Rat, Zebrafish |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Tubulin gamma Rabbit Monoclonal Antibody
View all gamma Tubulin Antibodies
SKU/Catalog Number
M06313
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Tubulin gamma Rabbit Monoclonal Antibody catalog # M06313. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Monkey, Mouse, Rat, Zebrafish.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Tubulin gamma Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06313)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DAE-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Tubulin gamma
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M06313 is reactive to TUBG1 in Human, Monkey, Mouse, Rat, Zebrafish
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
42 kDa
Calculated molecular weight
51170 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06313 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
IHC: human breast cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of Tubulin gamma expression in (1) HeLa cell lysate; (2) NIH 3T3 cell lysate; (3) C6 cell lysate; (4) Zebrafish lysates; (5) COS-1 cell lysate; (6) MDCK cell lysate.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human breast cancer, using Tubulin gamma Antibody.
Protein Target Info & Infographic
Gene/Protein Information For TUBG1 (Source: Uniprot.org, NCBI)
Gene Name
TUBG1
Full Name
Tubulin gamma-1 chain
Weight
51170 MW
Superfamily
tubulin family
Alternative Names
Tubulin gamma-1 chain;Gamma-1-tubulin;Gamma-tubulin complex component 1;GCP-1;TUBG1;TUBG; TUBG1 CDCBM4, GCP-1, TUBG, TUBGCP1 tubulin gamma 1 tubulin gamma-1 chain|gamma-tubulin complex component 1|tubulin, gamma polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TUBG1, check out the TUBG1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TUBG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Tubulin gamma Rabbit Monoclonal Antibody (M06313)
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Customer Q&As
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4 Customer Q&As for Anti-Tubulin gamma Rabbit Monoclonal Antibody
Question
Would anti-Tubulin gamma Rabbit Monoclonal antibody M06313 work on zebrafish IF with skin?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
Our lab technicians have not tested anti-Tubulin gamma Rabbit Monoclonal antibody M06313 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Tubulin gamma Rabbit Monoclonal antibody M06313 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish skin in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-04
Question
We are currently using anti-Tubulin gamma Rabbit Monoclonal antibody M06313 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
The anti-Tubulin gamma Rabbit Monoclonal antibody (M06313) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-24
Question
I see that the anti-Tubulin gamma Rabbit Monoclonal antibody M06313 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-07
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-07
Question
Will M06313 anti-Tubulin gamma Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-04-25
Answer
You can see on the product datasheet, M06313 anti-Tubulin gamma Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-04-25
