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Product Info Summary
| SKU: | M01493 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-CDK8 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01493
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CDK8 Rabbit Monoclonal Antibody catalog # M01493. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDK8 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01493)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GHF-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CDK8
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01493 is reactive to CDK8 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
60 kDa
Calculated molecular weight
53284 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01493 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
ICC/IF 1:50-1:500
IP 1:50
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CDK8 using anti-CDK8 antibody (M01493).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human MOLT-4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CDK8 antigen affinity purified monoclonal antibody (Catalog # M01493) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK8 at approximately 60 kDa. The expected band size for CDK8 is at 53 kDa.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:500 dilution.
Protein Target Info & Infographic
Gene/Protein Information For CDK8 (Source: Uniprot.org, NCBI)
Gene Name
CDK8
Full Name
Cyclin-dependent kinase 8
Weight
53284 MW
Superfamily
protein kinase superfamily
Alternative Names
Cyclin-dependent kinase 8;2.7.11.22;2.7.11.23;Cell division protein kinase 8;Mediator complex subunit CDK8;Mediator of RNA polymerase II transcription subunit CDK8;Protein kinase K35;CDK8; CDK8 IDDHBA, K35 cyclin dependent kinase 8 cyclin-dependent kinase 8|CDK8 protein kinase|cell division protein kinase 8|mediator complex subunit CDK8|mediator of RNA polymerase II transcription subunit CDK8|protein kinase K35
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDK8, check out the CDK8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDK8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CDK8 Rabbit Monoclonal Antibody (M01493)
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No publications found for M01493
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Customer Q&As
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6 Customer Q&As for Anti-CDK8 Rabbit Monoclonal Antibody
Question
My lab would like to test anti-CDK8 Rabbit Monoclonal antibody M01493 on mouse female gonad for research purposes, then I may be interested in using anti-CDK8 Rabbit Monoclonal antibody M01493 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
The products we sell, including anti-CDK8 Rabbit Monoclonal antibody M01493, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-30
Question
Can you help my question with product M01493, anti-CDK8 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01493 anti-CDK8 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-15
Question
Do you have a BSA free version of anti-CDK8 Rabbit Monoclonal antibody M01493 available?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CDK8 Rabbit Monoclonal antibody M01493 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-04
Question
Does anti-CDK8 Rabbit Monoclonal antibody M01493 work on canine IP with testis?
Verified Customer
Verified customer
Asked: 2019-04-25
Answer
Our lab technicians have not validated anti-CDK8 Rabbit Monoclonal antibody M01493 on canine. You can run a BLAST between canine and the immunogen sequence of anti-CDK8 Rabbit Monoclonal antibody M01493 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine testis in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-25
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for female gonad using anti-CDK8 Rabbit Monoclonal antibody M01493. Let me know if you need anything else.
M. Taylor
Verified customer
Asked: 2018-05-15
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-15
Question
Does anti-CDK8 Rabbit Monoclonal antibody M01493 work for WB with female gonad?
C. Moore
Verified customer
Asked: 2015-12-02
Answer
According to the expression profile of female gonad, CDK8 is highly expressed in female gonad. So, it is likely that anti-CDK8 Rabbit Monoclonal antibody M01493 will work for WB with female gonad.
Boster Scientific Support
Answered: 2015-12-02
