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Product Info Summary
| SKU: | A04047-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-MTUS1 Antibody Picoband®
SKU/Catalog Number
A04047-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MTUS1 Antibody Picoband® catalog # A04047-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MTUS1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04047-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MTUS1 recombinant protein (Position: D1038-N1236).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04047-1 is reactive to MTUS1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
170 kDa
Calculated molecular weight
23310 MW
Background of MTUS1
Mitochondrial tumor suppressor 1 (MTSG1) or Microtubule-Associated Scaffold Protein 1 (MTUS1) is a candidate tumor suppressor protein encoded by the MTUS1 gene in humans. It is mapped to 8p22. This gene encodes a protein which contains a C-terminal domain able to interact with the angiotension II (AT2) receptor and a large coiled-coil region allowing dimerization. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the transcript variants has been shown to encode a mitochondrial protein that acts as a tumor suppressor and partcipates in AT2 signaling pathways. Other variants may encode nuclear or transmembrane proteins but it has not been determined whether they also participate in AT2 signaling pathways.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04047-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human A549 whole cell, human HepG2 whole cell, human HEK293 whole cell, human Caco-2 whole cell, human Hela whole cell
ICC/IF: U20S cell
Validation Images & Assay Conditions
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Figure 1. IF analysis of MTUS1 using anti-MTUS1 antibody (A04047-1).
MTUS1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-MTUS1 Antibody (A04047-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 2. Western blot analysis of MTUS1 using anti-MTUS1 antibody (A04047-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human HEK293 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: human Hela whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTUS1 antigen affinity purified polyclonal antibody (Catalog # A04047-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTUS1 at approximately 170KD. The expected band size for MTUS1 is at 141KD.
Protein Target Info & Infographic
Gene/Protein Information For MTUS1 (Source: Uniprot.org, NCBI)
Gene Name
MTUS1
Full Name
Microtubule-associated tumor suppressor 1
Weight
23310 MW
Superfamily
MTUS1 family
Alternative Names
Microtubule-associated tumor suppressor 1; AT2 receptor-binding protein; Angiotensin-II type 2 receptor-interacting protein; Mitochondrial tumor suppressor 1; MTUS1; ATBP; ATIP; GK1; KIAA1288; MTSG1 MTUS1 ATBP, ATIP, ATIP3, ICIS, MP44, MTSG1 microtubule associated scaffold protein 1 microtubule-associated tumor suppressor 1|AT2 receptor-binding protein|AT2 receptor-interacting protein|AT2R binding protein|angiotensin-II type 2 receptor-interacting protein|erythroid differentiation-related|microtubule associated tumor suppressor 1|mitochondrial tumor suppressor gene 1|transcription factor MTSG1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MTUS1, check out the MTUS1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MTUS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MTUS1 Antibody Picoband® (A04047-1)
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Customer Q&As
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13 Customer Q&As for Anti-MTUS1 Antibody Picoband®
Question
I am interested in to test anti-MTUS1 antibody A04047-1 on rat lung for research purposes, then I may be interested in using anti-MTUS1 antibody A04047-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
The products we sell, including anti-MTUS1 antibody A04047-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-26
Question
Is there a BSA free version of anti-MTUS1 antibody A04047-1 available?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-MTUS1 antibody A04047-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-17
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-MTUS1 antibody A04047-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-12
Question
Does anti-MTUS1 antibody A04047-1 work for IF with lung?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
According to the expression profile of lung, MTUS1 is highly expressed in lung. So, it is likely that anti-MTUS1 antibody A04047-1 will work for IF with lung.
Boster Scientific Support
Answered: 2019-10-24
Question
I see that the anti-MTUS1 antibody A04047-1 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-30
Question
Would anti-MTUS1 antibody A04047-1 work on feline WB with corpus callosum?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
Our lab technicians have not validated anti-MTUS1 antibody A04047-1 on feline. You can run a BLAST between feline and the immunogen sequence of anti-MTUS1 antibody A04047-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline corpus callosum in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-27
Question
I have attached the WB image, lot number and protocol we used for lung using anti-MTUS1 antibody A04047-1. Please let me know if you require anything else.
T. Mangal
Verified customer
Asked: 2019-09-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-18
Question
Is a blocking peptide available for product anti-MTUS1 antibody (A04047-1)?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
We do provide the blocking peptide for product anti-MTUS1 antibody (A04047-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-17
Question
My question regarding product A04047-1, anti-MTUS1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-05-01
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04047-1 anti-MTUS1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-05-01
Question
Is this A04047-1 anti-MTUS1 antibody reactive to the isotypes of MTUS1?
D. Wu
Verified customer
Asked: 2017-03-22
Answer
The immunogen of A04047-1 anti-MTUS1 antibody is E.coli-derived human MTUS1 recombinant protein (Position: D1038-N1236). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-03-22
Question
Would A04047-1 anti-MTUS1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Li
Verified customer
Asked: 2016-07-19
Answer
It shows on the product datasheet, A04047-1 anti-MTUS1 antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-07-19
Question
I was wanting to use your anti-MTUS1 antibody for IF for rat lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat lung identification?
A. Lewis
Verified customer
Asked: 2014-12-17
Answer
It shows on the product datasheet, A04047-1 anti-MTUS1 antibody has been validated for ELISA, IF, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-12-17
Question
We are currently using anti-MTUS1 antibody A04047-1 for mouse tissue, and we are well pleased with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
O. Patel
Verified customer
Asked: 2014-06-17
Answer
The anti-MTUS1 antibody (A04047-1) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-06-17
