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Key=description, value=Mouse CCL22/MDC ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ccl22 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Key=short_description, value=Mouse CCL22/MDC ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ccl22 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Key=meta_keyword, value=Mouse MDC ELISA Kit, CCL22/MDC ELISA, Bosterbio High Sensitivity
Key=background, value=Macrophage-derived chemokine (MDC), also called Chemokine, cc motif, ligand 22 (CCL22) or Small inducible cytokine subfamily A, member 22 (SCY22). MDC is a recently identified member of the CC chemokine family. It is not closely related to other chemokines, sharing most similarity with thymus- and activation-regulated chemokine (TARC), which contains 37% identical amino acids. In addition, MDC gene is mapped to chromosome 16q13, the same position reported for the TARC gene. MDC has the four-cysteine motif and other highly conserved residues characteristic of CC chemokines, but it shares<35% identity with any of the known chemokines. Recombinant MDC was expressed in Chinese hamster ovary cells and purified by heparin-Sepharose chromatography. MDC is highly expressed in macrophages and in monocyte-derived dendritic cells, but not in monocytes, natural killer cells, or several cell lines of epithelial, endothelial, or fibroblast origin. High expression was also detected in normal thymus and less expression in lung and spleen. MDC is thus a unique member of the CC chemokine family that may play a fundamental role in the function of dendritic cells, natural killer cells, and monocytes.
Key=gene_name, value=Ccl22
Key=synonyms, value=C-C motif chemokine 22;Activated B and dendritic cell-derived;CC chemokine ABCD-1;Small-inducible cytokine A22;Ccl22;Abcd1, Scya22;
Key=sample_type, value=cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Key=kit_components, value=10 ng/tube
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  • Microplate reader in standard size.
  • Automated plate washer.
  • Incubator.
  • Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • Clean tubes and Eppendorf tubes.
  • Washing buffer (neutral PBS or TBS).
  • Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
  • Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

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Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)4623382052308871
Standard deviation2.9417.4850.844.1125.2666.20
CV (%)6.4%7.5%6.2%7.9%8.2%7.6%

Key=application_details, value=Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 31.2 pg/ml.
Key=subcellular_localization, value= Secreted.
Key=tissue_specificity, value= Expressed by activated splenic B-lymphocytes and dendritic cells. Low expression in lung, thymocytes, lymph node, and unstimulated splenic cells.
Key=protein_function, value= Chemotactic for activated T-lymphocytes. May play an important role in the collaboration of dendritic cells and B- lymphocytes with T-cells in immune responses.
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Concentration (pg/ml)031.262.512525050010002000
O.D.0.0160.0350.0460.0860.1860.5001.1462.011

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Key=immunogen, value=Expression system for standard: E.coli; Immunogen sequence: G25-S92
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LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 146515448493.036.1%
Sample 22332482342352376.102.5%
Sample 382085293689287543.484.9%
*number of samples for each test n=16.
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Mouse CCL22/MDC ELISA Kit PicoKine®

CCL22/MDC ELISA kit for Mouse

Mouse CCL22/MDC ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ccl22 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays. Cited in 9 publication(s).

Product Info Summary

SKU: EK0447
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse CCL22/MDC ELISA Kit PicoKine®

View all CCL22/MDC ELISA kits

SKU/Catalog Number

EK0447

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse CCL22/MDC ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ccl22 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse CCL22/MDC ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0447)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: E.coli; Immunogen sequence: G25-S92

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

See datasheet of EK0447 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0447 is reactive to Ccl22 in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0447 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of CCL22/MDC

Macrophage-derived chemokine (MDC), also called Chemokine, cc motif, ligand 22 (CCL22) or Small inducible cytokine subfamily A, member 22 (SCY22). MDC is a recently identified member of the CC chemokine family. It is not closely related to other chemokines, sharing most similarity with thymus- and activation-regulated chemokine (TARC), which contains 37% identical amino acids. In addition, MDC gene is mapped to chromosome 16q13, the same position reported for the TARC gene. MDC has the four-cysteine motif and other highly conserved residues characteristic of CC chemokines, but it shares<35% identity with any of the known chemokines. Recombinant MDC was expressed in Chinese hamster ovary cells and purified by heparin-Sepharose chromatography. MDC is highly expressed in macrophages and in monocyte-derived dendritic cells, but not in monocytes, natural killer cells, or several cell lines of epithelial, endothelial, or fibroblast origin. High expression was also detected in normal thymus and less expression in lung and spleen. MDC is thus a unique member of the CC chemokine family that may play a fundamental role in the function of dendritic cells, natural killer cells, and monocytes.

Kit Components

Catalog Number Description Quantity
EK0447-CAP Anti-Mouse Ccl22 Pre-coated 96-well strip microplate 1
EK0447-ST Mouse Ccl22 Standard 2 vials, 10 ng/tube
EK0447-DA Mouse Ccl22 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0160.0350.0460.0860.1860.5001.1462.011

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect CCL22/MDC, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 31.2 pg/ml..

Some articles we found to cite concentrations of CCL22/MDC in samples: 17961405 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse CCL22/MDC ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)4623382052308871
Standard deviation2.9417.4850.844.1125.2666.20
CV (%)6.4%7.5%6.2%7.9%8.2%7.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CCL22/MDC in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 146515448493.036.1%
Sample 22332482342352376.102.5%
Sample 382085293689287543.484.9%
*number of samples for each test n=16.

Gene/Protein Information For Ccl22 (Source: Uniprot.Org, NCBI)

Gene Name

Ccl22

Full Name

C-C motif chemokine 22

Weight

10302 MW

Superfamily

intercrine beta (chemokine CC) family

Alternative Names

C-C motif chemokine 22;Activated B and dendritic cell-derived;CC chemokine ABCD-1;Small-inducible cytokine A22;Ccl22;Abcd1, Scya22; CCL22 A-152E5.1, ABCD-1, DC/B-CK, MDC, SCYA22, STCP-1 C-C motif chemokine ligand 22 C-C motif chemokine 22|CC chemokine STCP-1|MDC(1-69)|chemokine (C-C motif) ligand 22|macrophage-derived chemokine|small inducible cytokine A22|small inducible cytokine subfamily A (Cys-Cys), member 22|stimulated T cell chemotactic protein 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Ccl22, check out the Ccl22 Infographic

Ccl22 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Ccl22: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

EK0447 has been cited in 9 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Fusion cytokine IL-2-GMCSF enhances anticancer immune responses through promoting cell–cell interactions

Systemic delivery of TNF-armed myxoma virus plus immune checkpoint inhibitor eliminates lung metastatic mouse osteosarcoma

Pathway analysis of global gene expression change in dendritic cells induced by the polysaccharide from the roots of Actinidia eriantha

Activation of RAW264.7 macrophages by active fraction of Albizia julibrissin saponin via Ca2+–ERK1/2–CREB–lncRNA pathways

Structural characteristics and immunopotentiation activity of two polysaccharides from the petal of Crocus sativus

He Y, Peng H, Zhang H, Liu Y, Sun H. Structural characteristics and immunopotentiation activity of two polysaccharides from the petal of Crocus sativus. Int J Biol Macromol. 2021 Mar 4:S0141-8130(21)00523-7. doi: 10.1016/j.ijbiomac.2021.03. 006. Epub ahead of print. PMID: 33676979.
Species: Mouse
EK0447 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELL, DILUTION:NA

Cao, Y., Cao, W., Qiu, Y., Zhou, Y., Guo, Q., Gao, Y., & Lu, N. (2020). Oroxylin A suppresses ACTN1 expression to inactivate cancer-associated fibroblasts and restrain breast cancer metastasis. Pharmacological Research, 104981.

Fusion cytokine IL-2-GMCSF enhances anticancer immune responses through promoting cell-cell interactions

Du, J., Chen, X., Wang, C., & Sun, H. (2017). Pathway analysis of global gene expression change in dendritic cells induced by the polysaccharide from the roots of Actinidia eriantha. Journal of Ethnopharmacology. Advance online publication. doi: 1...

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8 Customer Q&As for Mouse CCL22/MDC ELISA Kit PicoKine®

Question

Q: can I use citrate plasma as samples in Mouse CCL22 Picokine® ELISA Kit (Catalog # EK0447)?

Verified Customer

Verified customer

Asked: 2020-12-21

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of CCL22 causing disruption of its protein structure. CCL22 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the CCL22 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-12-21

Question

Q: if the enzyme conjugated CCL22 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CCL22 antigen?

Verified Customer

Verified customer

Asked: 2020-12-20

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-12-20

Question

Q: Can CCL22 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

W. Surve

Verified customer

Asked: 2020-09-17

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-09-17

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

L. Harris

Verified customer

Asked: 2020-08-21

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-08-21

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2020-06-26

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-06-26

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Z. King

Verified customer

Asked: 2018-07-03

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-07-03

Question

Q: What is the optimal O.D. value for CCL22 ELISA kit? I used your CCL22 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CCL22 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2018-02-21

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CCL22 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of CCL22 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2018-02-21

Question

Q: how to thaw whole blood sample for CCL22 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2016-06-29

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CCL22 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2016-06-29

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