Anti-TRPC6 Monoclonal Antibody

Product Name

Anti-TRPC6 Monoclonal Antibody

View all TRPC6 Antibodies

SKU/Catalog Number

M00625

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-TRPC6 Monoclonal Antibody (Catalog # M00625). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-TRPC6 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00625)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: 3F2.H10.F2 IgG1 kappa

Isotype

IgG1 kappa

Immunogen

This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human TRPC6 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

M00625 is reactive to TRPC6 in Human, Mouse

Reconstitution

Calculated molecular weight

106326 MW

Background of TRPC6

TRPC6, also known as TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6, is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC6 is probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. It is activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C and may not to be activated by intracellular calcium store depletion. Defects in this gene are a cause of focal segmental glomerulosclerosis (FSGS). Expression of this protein has been reported in tissues such as placenta, lung, spleen, ovary, small intestine, and renal podocytes. Immunohistochemistry studies using polyclonal antibodies to this target have shown moderate to strong staining in cell types such as neurons, breast, respiratory, squamous and prostate epithelium, epidermis, placental trophoblasts, dendritic cells, and subsets of immune cells, and faint to moderate staining of adrenal, colon, ganglion cells, hepatocytes, heart, and testis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

M00625 is guaranteed for ELISA, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:50,000
IHC: 2.5 µg/mL
WB: 1:500- 1:2,000

Validation Images & Assay Conditions

Gene/Protein Information For TRPC6 (Source: Uniprot.org, NCBI)

Alternative Names

TRPC6, TRPC 6, TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6 TRPC6 FSGS2, TRP6 transient receptor potential cation channel subfamily C member 6 short transient receptor potential channel 6|TRP-6|focal segmental glomerulosclerosis 2|transient receptor protein 6

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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