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Product Info Summary
| SKU: | EKC2174 |
|---|---|
| Size: | 1 kit, containing two 96-well plates and all necessary reagents |
| Reactive Species: | Human, Mouse, Rat |
| Application: | ELISA |
| Sample Types: | Cell lines |
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Product info
Product Name
PKC alpha (Phospho-Thr638) Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC2174
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The PKC alpha (Phospho-Thr638) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor PKC alpha (Phospho-Thr638) protein expression profile in cells. The kit can be used for measuring the relative amounts of PKC alpha (Phospho-Thr638) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PKC alpha (Phospho-Thr638).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
PKC alpha (Phospho-Thr638) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2174)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC2174 is reactive to PRKCA in Human, Mouse, Rat samples
Validated Sample Types
Cell lines
Application Guarantee
EKC2174 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 2 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-PKC alpha Phospho-Thr638 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Red |
| 100x Anti-PKC alpha Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 12ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 12ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 12ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from NIH-3T3 cells treated with UV 15'
Protein Target Info & Infographic
Gene/Protein Information For PRKCA (Source: Uniprot.Org, NCBI)
Gene Name
PRKCA
Full Name
Protein kinase C alpha type
Weight
76750 MW
Superfamily
protein kinase superfamily
Alternative Names
kinase PKC-alpha;KPCA;PKC III;PKC-A;PKC-alpha;PKC-III;PRKACA;PRKCA;Protein kinase C alpha type PRKCA AAG6, PKC-alpha, PKCA, PKCI+/-, PKCalpha, PRKACA protein kinase C alpha protein kinase C alpha type|PKC-A|aging-associated gene 6
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on PRKCA, check out the PRKCA Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PRKCA: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For PKC alpha (Phospho-Thr638) Colorimetric Cell-Based ELISA Kit (EKC2174)
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Customer Q&As
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1 Customer Q&As for PKC alpha (Phospho-Thr638) Colorimetric Cell-Based ELISA Kit
Question
I searches at your website and found EKC2174 PKC alpha (Phospho-Thr638) Colorimetric Cell-Based ELISA Kit. But I have a question about this product. Though the datasheet shows a western blot image showing it's binding to PKC-alpha, is the antibody tested for cross-reactivity with other members of PKC family like PKC-beta or PKC-delta.
Verified Customer
Verified customer
Asked: 2018-04-03
Answer
The antibody EKC2174 has not been tested for cross reactivity against other PKC family members. But in BLASTS and immunogen alignment showed that this product, has 85% and 66% homology against mouse PKC beta and gamma respectively. However, alignment against human demonstrate similar result, As such, it is not expected to have reaction with PKC gamma, yet there is good chance of reactivity against PKC beta.
Boster Scientific Support
Answered: 2018-04-03
