TRPC6 Antibodies

Short transient receptor potential channel 6 (TRPC6)

Thought to form a receptor-activated non-selective calcium permeant cation channel (PubMed:19936226, PubMed:23291369). Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors.

Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C (PubMed:26892346). Seems not to be activated by intracellular calcium store depletion.

Gene Information

Human
Gene Name:	TRPC6
Uniprot:	Q9Y210
Entrez:	7225

Family

Belongs to:
transient receptor (TC 1.A.4) family

Alternative Names

FLJ11098; FSGS2; short transient receptor potential channel 6; transient receptor potential cation channel, subfamily C, member 6; Transient receptor protein 6; TRP-6; TRP6FLJ14863; TrpC6

Molecular Weight

Mass (kDA):
106.326 kDA

Genomic Context

Human
Location:	11q22.1
Sequence:	11; NC_000011.10 (101451470..101584007, complement)

Tissue Specificity

Expressed primarily in placenta, lung, spleen, ovary and small intestine. Expressed in podocytes and is a component of the glomerular slit diaphragm.

Subcellular Localizations

Cell membrane; Multi-pass membrane protein.

Diseases

• Focal Glomerulosclerosis
• Hypertensive Disease
• Glomerulosclerosis (disorder)
• Kidney Diseases
• Segmental Glomerulosclerosis
• Pathologic Vasoconstriction
• Hypertrophy
• Proteinuria Of Undiagnosed Cause
• Nephrotic Syndrome
• Cardiac Hypertrophy
• Hypoxia
• Malignant Neoplasms
• Pulmonary Hypertension

• Neoplasms
• Diabetes Mellitus
• Stenosis
• Kidney Failure, Chronic
• Idiopathic Pulmonary Hypertension
• Renal Glomerular Disease
• Heart Failure

Interacting Proteins

• MX1

Pathways

• Pathogenesis
• Vasoconstriction
• Cell Proliferation
• Store-operated Calcium Entry
• Localization
• Membrane Depolarization
• Secretion
• Cell Growth
• Rna Interference
• Glomerular Filtration
• Cell Cycle
• Angiogenesis
• Cell Migration

• Platelet Activation
• Muscle Contraction
• Transport
• Reverse Transcription
• Cell Death
• Phototransduction
• Keratinocyte Differentiation

PTMs

• Phosphorylation
• Biotinylation
• Dephosphorylation
• Glycosylation
• Reduction
• Nitrosylation

• Hydroxylation
• Acylation
• Nitration
• Cleavage
• Acetylation
• Oxidation

• Myristoylation

Research Areas

• Signal Transduction

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/TRPC6

Boster Bio - Best Uses Of The TRPC6 Marker

If you're looking to make the most of your experiments, you might be wondering how to optimize the outcomes of your Boster Bio trials. Boster Bio's optimization guides are full of information to guide you on what to do, how to plan your experiments and how to ensure the most effective results. While every researcher will face problems during their experiments there are many possible causes that can be prevented. Below are a few of the most frequent sources of error and how to identify and eliminate them.

Boster Bio Anti-Macrosialin CD68 Antibody

The Boster Bio Anti-Macrosial Innate Immunocytochemistry (IHC) Monoclonal detection system is a high-performance antibody that was specifically designed for Human CD68. This antibody was tested on Human and Rabbit cells. It reacts with mouse and human cells in a variety immunological applications. It is made up of 5 mg BSA and 0.05mg thimerosal.

Macrosialin is used in numerous biological tests. Anti-Macrosialin antibodies, monoclonal as well as polyclonal react with Macrosialin on a variety of animal samples. The Boster Bio Anti-Macrosialin CD68 Antibody employs the TRPC6 marker to determine if the cell is infected with the protein. Although it isn't fully known, it is believed to be involved in tissue macrophage phagocytosis, intracellular lysosomal metabolism, and extracellular cell-cell interactions. It can be used to detect the substrates that contain selectins, and permit macrophages to walk over them.

The Boster Bio Anti-MacrosialIn CD68 Antibody detects the presence of CD68 in murine and human tissues. This glycoprotein that is highly glycosylated can be found in monocytes and macrophages as also in osteoclasts and myeloid-derived osteoclasts. The gene for CD68 is located at 17p13.1 and is expressed in the cytoplasmic and granules of blood cells. It serves as a reliable marker of the macrophage lineage. It can differentiate from other diseases with the same appearance.

Boster Bio Anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 6, Antibody

Transient Receptor Potential Cation Channel, (TRP) family members are membrane proteins which regulate various physiological processes in flies, humans, and worms. Boster Bio Anti -TRPC6 Antibody recognizes the protein in human, mouse or the cells of rat. The Boster Bio Anti-TRPC6 Antibody recognizes TRP6 and at least five other proteins. The antibody is tested in WB using mouse and rat tissues.

Research grade antibodies are produced after months of research and benchwork in order to determine the specificity of an anti-matter protein antibody. The antibodies are then purified and characterised. The products that are created undergo validation to ensure they meet the strict criteria of efficacy and specificity. Commercially available antibodies can be purchased for less price than those available to academics.

The Boster Anti-Transient Receptor Potential C–Channel, Subfamily C Member 6 Antibody is suitable when you are a researcher. Your results can be submitted for research that is based on application and specific to species. The resulting publications may receive product credits. The Boster Anti Transient Receptor Potentially CChain Subfamily C Member 6 Antibody is designed for research purposes only. It is not intended to be used in diagnostic procedures or resold for sale without prior authorization.

The TRPC6 protein is a membrane-based calcium channel that is activated by a receptor. It is controlled by the phosphatidylinositol secondary messenger system, which is independent of protein kinase C. Additionally that, low intracellular levels of calcium do not trigger activation of the TRPC6 channel. Additionally, defects in the TRPC6 gene can cause focal segment glomerulosclerosis, as well as ophthalmic kidney disease.

Boster Bio Anti-DAG

Boster Bio Anti–DAG antibody was tested against the TRPC6 marker in human and mouse tissues. The antibody reacts with human, mouse, and rat tissues. In addition, it has no cross-reactivity to other proteins. Boster Bio Anti-DAG was developed to detect DAGs within human tissues. It is suitable for multiplex ELISA, Immunofluorescence and WB applications.

The TRPC6 marker is a molecular target utilized to aid in diagnosis, is widely employed. In-situ hybridization was carried out by using 3' digoxigenin labeled antisense Oligonucleotides. These were obtained from Boster in Wuhan, China. The Boster Bio Anti-DAG employs the TRPC6 marker, as well as a biotinylated antidigoxin mouse antibody. The markers were identified using the resulting signals.

The TRPC6 marker is an essential biological marker for toxic substances related to DAG. In this study, we found that the PIP2 marker decreased the association between TRPC6 and PIP2. This could be due to the inhibitory actions of PIP2 on TRPC6. Boster Bio Anti-DAG employs the TRPC6 marker as a way to identify DAG-producing cells.

With helper plasmids lentiviral or shRNA-expressing vectors, we stably transduced 293T cells. We then used these viral vectors or shRNAs to infect the target cells. After 48 hours, we isolated cells which had been transduced by shRNA. We also conducted western analysis to confirm knockdown of TRPC6.

Boster Bio ELISA Kits

ELISA is a helpful instrument to measure the levels of soluble proteins. Researchers can make use of the reagents to determine the levels of proteins in their native state. This molecule can be identified using a variety of biological assays that use antibodies. Boster Bio developed antibodies that react with proteins from various animals. They are specific to rabbits and mice. Boster Bio offers a complete procedure to assist you in preparing your sample to get the optimal results.

Enzyme-linked immunosorbent assays are plate-based tests that detect proteins or peptides. To perform an ELISA the antigen needs to be immobilized on a surface and then bound with a specific antibody. After the two reagents have been incubated and the enzyme is released, it will produce an identifiable product. High-affinity antibodies are crucial for the assay's accuracy and sensitivity.

Boster Bio Optimization Tips

Boster Bio provides a wide selection of isotype-specific controls and secondary antibodies for IHC. Boster Bio also offers IHC-optimized detection systems. Here are some frequently asked questions. These are answered in the product datasheets. Find out more about the use of Boster Bio products for IHC. If you have any questions about this particular marker, you can find answers to them in the product datasheets.