Product Info Summary
SKU: | A13306 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Synaptotagmin-16 SYT16 Antibody
SKU/Catalog Number
A13306
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Synaptotagmin-16 SYT16 Antibody (Catalog # A13306). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Synaptotagmin-16 SYT16 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13306)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human SYT16.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A13306 is reactive to SYT16 in Human
Reconstitution
Calculated molecular weight
72274 MW
Background of SYT16
May be involved in the trafficking and exocytosis of secretory vesicles in non-neuronal tissues. Is Ca2+-independent.
Fukuda M., J. Biochem. 133:641-649(2003).
Craxton M.A., BMC Genomics 5:43-43(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13306 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from K562 cells, Jurkat cells, 293 cells and HepG2 cells, using SYT16 antibody A13306.
Protein Target Info & Infographic
Gene/Protein Information For SYT16 (Source: Uniprot.org, NCBI)
Gene Name
SYT16
Full Name
Synaptotagmin-16
Weight
72274 MW
Superfamily
synaptotagmin family
Alternative Names
Synaptotagmin-16;Chr14Syt;Synaptotagmin 14-like protein;Synaptotagmin XIV-related protein;SYT16;STREP14, SYT14L, SYT14R; SYT16 CHR14SYT, SYT14L, Strep14, syt14r synaptotagmin 16 synaptotagmin-16|chr14 synaptotagmin|synaptotagmin 14-like protein|synaptotagmin XIV-related protein|synaptotagmin XVI
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SYT16, check out the SYT16 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SYT16: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Synaptotagmin-16 SYT16 Antibody (A13306)
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No publications found for A13306
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Customer Q&As
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6 Customer Q&As for Anti-Synaptotagmin-16 SYT16 Antibody
Question
I was wanting to use your anti-SYT16 antibody for WB for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
As indicated on the product datasheet, A13306 anti-SYT16 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-31
Question
Here is the WB image, lot number and protocol we used for brain using anti-SYT16 antibody A13306. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-17
Question
We are currently using anti-SYT16 antibody A13306 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
The anti-SYT16 antibody (A13306) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-07
Question
My question regarding product A13306, anti-SYT16 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-11-08
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A13306 anti-SYT16 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-11-08
Question
Will A13306 anti-SYT16 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-08-14
Answer
As indicated on the product datasheet, A13306 anti-SYT16 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-08-14
Question
Is a blocking peptide available for product anti-SYT16 antibody (A13306)?
K. Krishna
Verified customer
Asked: 2017-05-11
Answer
We do provide the blocking peptide for product anti-SYT16 antibody (A13306). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-05-11