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Product Info Summary
| SKU: | M00591 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, ICC |
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Product info
Product Name
Anti-Hu CD71 Purified TFRC Monoclonal Antibody
SKU/Catalog Number
M00591
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD71 Purified TFRC Monoclonal Antibody (Catalog# M00591). Tested in Flow Cytometry, IP, ICC application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD71 Purified TFRC Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00591)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
MEM-75
Isotype
Mouse IgG1
Immunogen
NALM-6 human pre-B cell line. The antibody MEM-75 reacts with an extracellular epitope of CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and T lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes. The antibody MEM-75 does not block binding of transferrin to the receptor.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00591 is reactive to TFRC in Human
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
84871 MW
Background of TFRC
CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00591 is guaranteed for Flow Cytometry, IP, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 0.5-4 μg/ml.
Validation Images & Assay Conditions
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Figure 1. Immunocytochemistry staining of TFRC using Anti-Hu CD71 Purified TFRC Monoclonal Antibody (M00591).
Immunocytochemistry staining of CD71 in human HeLa cell line using anti-CD71 (MEM-75; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).Protein Target Info & Infographic
Gene/Protein Information For TFRC (Source: Uniprot.org, NCBI)
Gene Name
TFRC
Full Name
Transferrin receptor protein 1
Weight
84871 MW
Superfamily
peptidase M28 family
Alternative Names
TfR1, TfR, TR, Trfr, T9, p90, TFRC TFRC CD71, IMD46, T9, TFR, TFR1, TR, TRFR, p90 transferrin receptor transferrin receptor protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TFRC, check out the TFRC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TFRC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hu CD71 Purified TFRC Monoclonal Antibody (M00591)
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