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Product Info Summary
| SKU: | M00094 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-Transferrin TF Rabbit Monoclonal Antibody
View all Transferrin Antibodies
SKU/Catalog Number
M00094
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Transferrin TF Rabbit Monoclonal Antibody catalog # M00094. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Transferrin TF Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00094)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DOH-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Transferrin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00094 is reactive to TF in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
52 kDa
Calculated molecular weight
77064 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00094 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
FC 1:50
Positive Control
WB: Human serum
IHC: Human breast carcinoma tissue
Validation Images & Assay Conditions
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Western blot analysis of Transferrin expression in Human serum lysate.
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Immunohistochemical analysis of paraffin-embedded Human breast carcinoma, using Transferrin Antibody.
Protein Target Info & Infographic
Gene/Protein Information For TF (Source: Uniprot.org, NCBI)
Gene Name
TF
Full Name
Serotransferrin
Weight
77064 MW
Superfamily
transferrin family
Alternative Names
Serotransferrin;Transferrin;Beta-1 metal-binding globulin;Siderophilin;TF;PRO1400; TF HEL-S-71p, PRO1557, PRO2086QTL1, TF transferrin serotransferrin|beta-1 metal-binding globulin|epididymis secretory sperm binding protein Li 71p|siderophilin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TF, check out the TF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Transferrin TF Rabbit Monoclonal Antibody (M00094)
Hello CJ!
M00094 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
MCPIP is induced by cholesterol and participated in cholesterol-caused DNA damage in HUVEC
Liang W, Zhang W, Zhao S, Li Q, Yang Y, Liang H, Ceng R. Mol Med Rep. 2015 Feb;11(2):903-9. Doi: 10.3892/Mmr.2014.2822. Epub 2014 Oct 30. A Study Of The Ultrasound-Targeted Microbubble Destruction Based Triplex-Forming Oligodexinucleotide Delivery...
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Transferrin TF Rabbit Monoclonal Antibody
Question
Is a blocking peptide available for product anti-Transferrin Rabbit Monoclonal antibody (M00094)?
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
We do provide the blocking peptide for product anti-Transferrin Rabbit Monoclonal antibody (M00094). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-02
Question
I see that the anti-Transferrin Rabbit Monoclonal antibody M00094 works with IP, what is the protocol used to produce the result images on the product page?
R. Dhar
Verified customer
Asked: 2020-02-03
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-03
Question
We are currently using anti-Transferrin Rabbit Monoclonal antibody M00094 for human tissue, and we are satisfied with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
The anti-Transferrin Rabbit Monoclonal antibody (M00094) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-29
Question
Is there a BSA free version of anti-Transferrin Rabbit Monoclonal antibody M00094 available?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Transferrin Rabbit Monoclonal antibody M00094 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-22
Question
See below the WB image, lot number and protocol we used for brain using anti-Transferrin Rabbit Monoclonal antibody M00094. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-31
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Transferrin Rabbit Monoclonal antibody M00094. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-05
Question
Would M00094 anti-Transferrin Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
As indicated on the product datasheet, M00094 anti-Transferrin Rabbit Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-23
Question
you antibody to test anti-Transferrin Rabbit Monoclonal antibody M00094 on human brain for research purposes, then I may be interested in using anti-Transferrin Rabbit Monoclonal antibody M00094 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
The products we sell, including anti-Transferrin Rabbit Monoclonal antibody M00094, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-15
Question
We bought anti-Transferrin Rabbit Monoclonal antibody for WB on brain in the past. I am using human, and I plan to use the antibody for IP next. Our lab want to know about examining brain as well as heart in our next experiment. Do you have any suggestion on which antibody would work the best for IP?
S. Evans
Verified customer
Asked: 2019-07-18
Answer
I viewed the website and datasheets of our anti-Transferrin Rabbit Monoclonal antibody and it seems that M00094 has been tested on human in both WB and IP. Thus M00094 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-18
Question
Is this M00094 anti-Transferrin Rabbit Monoclonal antibody reactive to the isotypes of TF?
D. Krishna
Verified customer
Asked: 2019-05-15
Answer
The immunogen of M00094 anti-Transferrin Rabbit Monoclonal antibody is A synthesized peptide derived from human Transferrin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-15
Question
We have been able to see staining in mouse inferior vagus x ganglion. Do you have any suggestions? Is anti-Transferrin Rabbit Monoclonal antibody supposed to stain inferior vagus x ganglion positively?
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
According to literature inferior vagus x ganglion does express TF. According to Uniprot.org, TF is expressed in inferior vagus x ganglion, liver, brain, fetal liver, brain, cajal-retzius cell fetal brain cortex, heart, bile, plasma, saliva, cerebrospinal fluid, among other tissues. Regarding which tissues have TF expression, here are a few articles citing expression in various tissues:
Bile, Pubmed ID: 15084671
Brain, Pubmed ID: 9272172, 15489334
Cerebrospinal fluid, Pubmed ID: 19838169
Heart, Pubmed ID: 7498159
Liver, Pubmed ID: 1809186, 19159218, 24275569
Plasma, Pubmed ID: 14760718, 16335952
Saliva, Pubmed ID: 16740002
Boster Scientific Support
Answered: 2019-05-15
Question
My team were well pleased with the WB result of your anti-Transferrin Rabbit Monoclonal antibody. However we have been able to see positive staining in fetal liver secreted. using this antibody. Is that expected? Could you tell me where is TF supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-02-13
Answer
From literature, fetal liver does express TF. Generally TF expresses in secreted. Regarding which tissues have TF expression, here are a few articles citing expression in various tissues:
Bile, Pubmed ID: 15084671
Brain, Pubmed ID: 9272172, 15489334
Cerebrospinal fluid, Pubmed ID: 19838169
Heart, Pubmed ID: 7498159
Liver, Pubmed ID: 1809186, 19159218, 24275569
Plasma, Pubmed ID: 14760718, 16335952
Saliva, Pubmed ID: 16740002
Boster Scientific Support
Answered: 2018-02-13
Question
Does anti-Transferrin Rabbit Monoclonal antibody M00094 work for IP with brain?
Verified Customer
Verified customer
Asked: 2017-07-25
Answer
According to the expression profile of brain, TF is highly expressed in brain. So, it is likely that anti-Transferrin Rabbit Monoclonal antibody M00094 will work for IP with brain.
Boster Scientific Support
Answered: 2017-07-25
Question
I have a question about product M00094, anti-Transferrin Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
H. Zhao
Verified customer
Asked: 2016-10-11
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00094 anti-Transferrin Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-10-11
Question
I was wanting to use your anti-Transferrin Rabbit Monoclonal antibody for IP for human brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brain identification?
K. Bhatt
Verified customer
Asked: 2013-06-03
Answer
As indicated on the product datasheet, M00094 anti-Transferrin Rabbit Monoclonal antibody has been validated for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-06-03
