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Product Info Summary
| SKU: | A02805-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Transcription factor Spi-B SPIB Antibody
SKU/Catalog Number
A02805-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Transcription factor Spi-B SPIB Antibody (Catalog # A02805-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
SPIB antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Transcription factor Spi-B SPIB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02805-1)
Host
Rabbit
Contents
SPIB antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SPIB antibody was raised against a 17 amino acid peptide near the carboxy terminus of human SPIB. The immunogen is located within amino acids 200 - 250 of SPIB.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
SPIB antibody is human, mouse and rat reactive. At least four isoforms of SPIB are known to exist; this antibody will detect all but isoform 3.
Reactive Species
A02805-1 is reactive to SPIB in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
28819 MW
Background of Spi-B
SPIB is a member of the ETS transcription factor family that influences lymphoid development and activity and binds the consensus DNA site GGA (A/T) through a unique winged helix-turn-helix motif known as the ETS domain (1). SPIB is found in hematopoietic cells such as B cells and plasmacytoid dendritic cells (DC) (2,3). It promotes the development of plasmacytoid dendritic cells (pDCs) or natural interferon (IFN)-producing cells. SPIB may be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation (3,4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02805-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SPIB antibody can be used for detection of SPIB by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SPIB in human prostate tissue lysate with SPIB antibody at 1 μg/ml.
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Immunohistochemistry of SPIB in human prostate tissue with SPIB antibody at 2.5 μg/mL.
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Immunohistochemistry of SPIB in human prostate tissue with SPIB antibody at 2.5 μg/ml.
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Immunofluorescence of SPIB in human prostate tissue with SPIB Antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For SPIB (Source: Uniprot.org, NCBI)
Gene Name
SPIB
Full Name
Transcription factor Spi-B
Weight
28819 MW
Superfamily
ETS family
Alternative Names
Spi-B transcription factor, SPI-B SPIB SPI-B Spi-B transcription factor transcription factor Spi-B|Spi-B transcription factor (Spi-1/PU.1 related)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SPIB, check out the SPIB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SPIB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Transcription factor Spi-B SPIB Antibody (A02805-1)
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