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Product Info Summary
| SKU: | A02805 |
|---|---|
| Size: | 100 µg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Spi-B Antibody
SKU/Catalog Number
A02805
Size
100 µg
Form
Liquid
Description
Boster Bio Anti-Spi-B Antibody (Catalog# A02805). Tested in IHC, WB application(s). This antibody reacts with Human.
Storage & Handling
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Cite This Product
Anti-Spi-B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02805)
Host
Rabbit
Contents
100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A partial length recombinant Spi-B protein (amino acids 50-260)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A02805 is reactive to SPIB in Human
Reconstitution
Calculated molecular weight
28819 MW
Background of Spi-B
FANCC (also called Protein FACC or Fanconi Anemia Group C protein) is involved in DNA repair, perhaps specifically with post-replication repair or a cell cycle checkpoint function. FANCC may also be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. FANCC belongs to the multi-subunit Fanconi Anemia (FA) complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM. FANCC is mainly found within the nucleus although some protein is localized in the cytoplasm. This protein is ubiquitously expressed. Defects in FANCC are a cause of Fanconi anemia (FA). FA is a genetically heterogeneous, autosomal recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group C.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02805 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot: 2-4 µg/ml
IHC: 5 µg/ml
Validation Images & Assay Conditions
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Immunohistochemical analysis of Spi-B. Anti-Spi-B antibody in human Colon tissue at 5 &mu
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Western blot analysis of Spi-B. Anti-Spi-B antibody was used at 4 &mu
Protein Target Info & Infographic
Gene/Protein Information For SPIB (Source: Uniprot.org, NCBI)
Gene Name
SPIB
Full Name
Transcription factor Spi-B
Weight
28819 MW
Superfamily
ETS family
Alternative Names
Transcription factor Spi-B;SPIB; SPIB SPI-B Spi-B transcription factor transcription factor Spi-B|Spi-B transcription factor (Spi-1/PU.1 related)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SPIB, check out the SPIB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SPIB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Spi-B Antibody (A02805)
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No publications found for A02805
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Customer Q&As
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1 Customer Q&As for Anti-Spi-B Antibody
Question
We are currently using anti-Spi-B antibody A02805 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
The anti-Spi-B antibody (A02805) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-02
