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Product Info Summary
| SKU: | PROTQ8K3I6 |
|---|---|
| Size: | 2ug, 10ug, 1mg |
| Origin Species: | Mouse |
| Source: | Escherichia coli |
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Product info
Product Name
IL-27 Interleukin-27 Mouse Recombinant Protein
View all IL27 recombinant proteins
SKU/Catalog Number
PROTQ8K3I6
Size
2ug, 10ug, 1mg
Description
Interleukin-27 Mouse Recombinant produced in E. coli is a single, non-glycosylated polypeptide chain containing 207 amino acids and having a molecular mass of 23.7 kDa. The Murine IL-27 is purified by proprietary chromatographic techniques.
Storage & Handling
Lyophilized IL-27 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL27 should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Cite This Product
IL-27 Interleukin-27 Mouse Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTQ8K3I6)
Form
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from 10mM NaHCO₃ buffer pH-8.5.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Predicted MW
27.493kDa
Reconstitution
It is recommended to reconstitute the lyophilized IL-27 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Amino Acid Sequence
MFPTDPLSLQ ELRREFTVSL YLARKLLSEV QGYVHSFAES RLPGVNLDLL PLGYHLPNVS LTFQAWHHLS DSERLCFLAT TLRPFPAMLG GLGTQGTWTS SEREQLWAMR LDLRDLHRHL RFQVLAAGFK CSKEEEDKEE EEEEEEEEKK LPLGALGGPN QVSSQVSWPQ LLYTYQLLHS LELVLSRAVR DLLLLSLPRR PGSAWDS
Biological Activity
Mouse p28 biological activity was measured via dose-dependent inhibition of TGF-beta and IL-6-induced IL-17A expression in mouse CD4 splenocytes. 50ng/ml of mouse p28 is capable of inhibiting greater than 25% of IL-17A expression in this assay.
Assay dilution & Images
Reconstitution
It is recommended to reconstitute the lyophilized IL-27 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Validation Images & Assay Conditions
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Recombinant protein fun image
Protein Target Info & Infographic
Gene/Protein Information For IL27 (Source: Uniprot.org, NCBI)
Gene Name
IL27
Full Name
Interleukin-27 subunit alpha
Weight
27.493kDa
Superfamily
IL-6 superfamily
Alternative Names
Interleukin-30; IL-30; IL-27/p28; p28; Interleukin-27; Interleukin-27/p28; IL-27; Interleukin-27 subunit alpha; IL-27 subunit alpha; IL27-A; Il27; Il27a; IL-27p28 IL27 IL-27, IL-27AA, IL27p28, IL30, p28, IL27 interleukin 27 interleukin-27 subunit alpha|IL-27 p28 subunit|IL-27 subunit alpha|IL-27-A|IL27-A|interleukin-30
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on IL27, check out the IL27 Infographic
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In this infographic, you will see the following information for IL27: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For IL-27 Interleukin-27 Mouse Recombinant Protein (PROTQ8K3I6)
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