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Product Info Summary
| SKU: | A03068-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Transcription factor E2F3 E2F3 Antibody
SKU/Catalog Number
A03068-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Transcription factor E2F3 E2F3 Antibody (Catalog # A03068-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
E2F3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Transcription factor E2F3 E2F3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03068-1)
Host
Rabbit
Contents
E2F3 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E2F3 antibody was raised against a 15 amino acid peptide near the center of human E2F3. The immunogen is located within amino acids 150 - 200 of E2F3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
E2F3 antibody is human and mouse reactive. At least three isoforms of E2F3 are known to exist; this antibody will detect only the largest isoform. This antibody is predicted to not cross-react with other members of the E2F transcription factor family.
Reactive Species
A03068-1 is reactive to E2F3 in Human, Mouse
Applications
A03068-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
49162 MW
Background of E2F3
The E2F transcription factor 3 (E2F3) is a member of a small family of transcription factors that function through binding of DP interaction partner proteins. E2F3 recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle (1). Like the related E2F1 and E2F2, E2F3 is essential for cellular proliferation and progression through the cell cycle (2). Altered copy number and activity of this gene have been observed in a number of human cancers (3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
E2F3 antibody can be used for detection of E2F3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of E2F3 in human lymph node tissue lysate with E2F3 antibody at 1 μg/ml.
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Immunohistochemistry of E2F3 in human lymph node tissue with E2F3 antibody at 5 μg/ml.
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Immunofluorescence of E2F3 in human lymph node tissue with E2F3 antibody at 20 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For E2F3 (Source: Uniprot.org, NCBI)
Gene Name
E2F3
Full Name
Transcription factor E2F3
Weight
49162 MW
Superfamily
E2F/DP family
Alternative Names
DKFZp686C18211; E2F transcription factor 3; E2F-3; KIAA0075; MGC104598; transcription factor E2F3 E2F3 E2F-3 E2F transcription factor 3 transcription factor E2F3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on E2F3, check out the E2F3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for E2F3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Transcription factor E2F3 E2F3 Antibody (A03068-1)
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