Transcription factor E2F3 Antibodies

Transcription factor E2F3 (E2F3)

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a variety of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase.

E2F3 binds specifically to RB1 in a cell-cycle dependent way. Inhibits adipogenesis, likely through the repression of CEBPA binding to its target gene promoters (By similarity).

Gene Information

Human
Gene Name:	E2F3
Uniprot:	O00716
Entrez:	1871

Family

Belongs to:
E2F/DP family

Alternative Names

DKFZp686C18211; E2F transcription factor 3; E2F-3; KIAA0075; MGC104598; transcription factor E2F3

Molecular Weight

Mass (kDA):
49.162 kDA

Genomic Context

Human
Location:	6p22.3
Sequence:	6; NC_000006.12 (20401879..20493714)

Subcellular Localizations

Nucleus.

Diseases

• Neoplasms
• Retinoblastoma
• Malignant Neoplasms
• Carcinoma
• Cell Transformation, Neoplastic
• Hyperplasia
• Malignant Neoplasm Of Prostate
• Prostatic Neoplasms
• Malignant Neoplasm Of Breast
• Bladder Neoplasm
• Malignant Neoplasm Of Urinary Bladder
• Malignant Paraganglionic Neoplasm

• Neoplasm Metastasis
• Carcinogenesis
• Malignant Neoplasm Of Ovary
• Ovarian Neoplasm
• Mammary Neoplasms
• Tumor Progression
• Lung Neoplasms

Interacting Proteins

• HELLS
• L3MBTL3
• MSH2
• RB1

Pathways

• Cell Cycle
• Cell Proliferation
• Cell Growth
• S Phase
• Dna Replication
• Mitosis
• Cell Cycle Arrest
• Cell Division
• Senescence
• Methylation
• Dna Repair
• Induction Of Apoptosis
• Cell Death

• Pathogenesis
• Translation
• Localization
• Oncogenesis
• G1 Phase
• Rna Interference
• Cell Differentiation

PTMs

• Phosphorylation
• Methylation

• Acetylation
• Ubiquitination

• Dephosphorylation

Research Areas

• Cell Cycle and Replication
• Core ESC-Like Genes
• Stem Cell Markers

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/E2F3

Best Uses of the E2F3 Marker

You can get more details about this biomarker in this article. You'll learn about how you can use this product for ICC and Immunofluorescence and the many applications it can serve in cancer research.

Anti-Transcription Factor E2F3

Bioinformatics analysis has revealed that lung cancer tissues express the anti-Transcription E2F3 protein in a different manner from non-tumor lung tissue. These results were confirmed by tissue microarray analysis and the development of a lung cancer-specific cell-line. In addition, 19 studies were conducted to further study E2F3's role in lung cancer and its relation to the disease.

Transcription factor E2F3 belongs to the E2F family of. It plays a significant role in numerous cellular processes. These include cell division DNA replication, cell cycle, and Apoptosis. Each member of the E2F family has distinct effects on the growth of cancer cells. It is classified as an activator of transcription or a transcriptional repressor. Furthermore, E2F3 has been shown to interact with the histone acetyltransferase.

The ELISA assay results show that this marker is positive for anti-Transcription Factor E2F-3. This indicates that the anti Transcription Factor E2F-3 antibody is specifically targeted to E2F3 tumor cells. Boster Bio Anti-Transcription Factor E2F3 antibody forms a part of the Picoband(tm) catalog and is able to react with Human, Mouse, Rat and frog. It can be stored at -20°C for up to one year. Boster Bio Anti-Transcription factor E2F3 antibody comprises Trehalose and Na2HPO4, C-terminal sequence and Na2HPO4.

Another transcription factor that targets E2F3 is MiR-34a (miR-34) The binding site is located in the 3'UTR region of E2F3. The miR34a binds E2F3 in mice, humans and rat genomes. Inhibition of E2F activity hinders myeloid cell proliferative.

ICC

Non-member countries and international organizations have backed the International Criminal Court's efforts to establish a permanent court. The United States cooperated with Rwanda to transfer Bosco Ntaganda (a suspect in a case that was filed by the ICC). Bosco Ntaganda was able to surrender himself to the United States Embassy in Rwanda, regardless of the fact that he was not a resident of the United States. The United States also supported ICC efforts by adopting a binding policy in support of the establishment and operation of the court. The United States of America and the European Union, although they aren't ICC members in the past, have provided financial assistance and are currently the most significant source of funding for the court. In the United Nations General Assembly, the organization approves additional funds for cases that are referred to it by the Security Council. Certain governments also offer

The guidelines for international competitions that use the E2F3 marker were developed by the ICC. The E2F3 marker is now one of the most popular kinds of international sport marker. This marker is used to identify irregularities in ICC events. Its use is increasingly popular especially in the United States. Its use has a variety of applications and is used in international sports, from video game tournaments and high-profile sporting events.

The E2F3 gene is one of the most important members of the E2F protein family that regulates the expression of genes and the cell cycle, is commonly referred to as the E2F3 gene. It is also necessary to fully activate the MYCN promoter in the NB. Thus, an increase in the transcription factor encoded by the MYCN gene is crucial for controlling cell cycle, apoptosis, and blocking differentiation in primary neural crest cells.

Immunofluorescence

To test the efficacy of E2F3 as a biomarker of lung cancer, a bioinformatics study was conducted. The study revealed a differential expression of E2F3 in lung cancer tissue as compared to non-tumor tissues surrounding it. Additionally, it confirmed the results of an organ microarray and a cell line of patients with NSCLC. Additionally 19 studies were conducted to evaluate the role of E2F3 for lung cancer, including analyses in the Cancer Genome Atlas.

To determine the expression levels of DNA or RNA antibodies against E2F3 were employed. Boster Bio's E2F3 monoclonal antibody for immunofluorescence reacts with the RNA-derived E2F3 proteins. This monoclonal antibody can be kept at -20C for 1 year. It comes in PBS containing 0.02 sodium azide.

E2F3 mRNA and protein are produced by myoblasts, but are suppressed in proliferating cells. E2F3 levels that are lower than normal hinder the transcription of cell-cycle genes which can lead to an interruption of G1-phage activity and hinder differentiation. MyoG is a myogenic marker in cells that are undergoing differentiation and decreased E2F3 expression reduces myogenic gene expression.

NSCLC

The E2F3 marker in N SCLC has the greatest applications for early detection of lung cancer as well as prediction of the disease's outcome. This marker has been shown to be strongly linked with the outcome of lung cancer and is a promising candidate for early detection. The study found that E2F3 positive detection and tumor response markers were both associated with cancer response and PFS.

The response to chemotherapy in colorectal and gastric cancer has been related to tumor-related markers. However the use of tumor markers in advanced stages of NSCLC is limited, with only one study evaluating the importance of seven markers for tumors in relation to the prognosis as well as the response to palliative therapy. In this study, the patients with the highest levels of tumor markers had a better prognosis than those with normal levels of the remaining markers.

The E2F3 gene is essential to the development of a variety of cancer. Recent research has focused on studying the function of the E2F3 gene in lung cancer and comparing its expression with that of nearby non-tumor tissue. In addition, these researchers examined the bioinformatics and biomarkers of the E2F3 gene in a lung cancer cell line and a tissue microarray. This was then verified. The significance of the E2F3 Gene in NSCLC was also assessed and its integration into the Cancer Genome Atlas database.

A study of the E2F3 gene discovered that the protein was linked to the spread and progression of lung cancer. The study showed that tumor cells can become overexpressed of the Bcl-2 antipoptotic gene E2F3 and this could result in uncontrolled growth. This gene could be a valuable biomarker for NSCLC.

The Best Uses for the E2F3 Marker in N-Small Cell Lung Cancer