Anti-MRPL42 Antibody

Product Name

Anti-MRPL42 Antibody

View all MRPL42 Antibodies

SKU/Catalog Number

A13167-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-MRPL42 Antibody catalog # A13167-1. Tested in WB applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MRPL42 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13167-1)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human MRPS32.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A13167-1 is reactive to MRPL42 in Human, Mouse, Rat

Applications

A13167-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

16661 MW

Background of MRPL42

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a protein identified as belonging to both the 28S and the 39S subunits. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 4q, 6p, 6q, 7p, and 15q.

Mao Y.F., Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases.
Wiemann S., Genome Res. 11:422-435(2001).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1000

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blotting validation for Anti-MRPL42 Antibody A13167-1

Western blot (WB) analysis of MRPL42 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate
Lane2:Raw264.7 whole cell lysate
Lane3:H9C2 whole cell lysate
Lane4:HELA whole cell lysate
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For MRPL42 (Source: Uniprot.org, NCBI)

Alternative Names

39S ribosomal protein L31, mitochondrial; 39S ribosomal protein L42, mitochondrial; HSPC204,28S ribosomal protein S32, mitochondrial; L31mt; mitochondrial ribosomal protein L42; mitochondrial ribosomal protein S32; MRPL31L42mt; MRP-L31MRP-S32; MRPS32MRP-L42; PTD007; RPML31S32mt MRPL42 HSPC204, L31MT, L42MT, MRP-L31, MRP-L42, MRP-S32, MRPL31, MRPS32, PTD007, RPML31, S32MT mitochondrial ribosomal protein L42 39S ribosomal protein L42, mitochondrial|28S ribosomal protein S32, mitochondrial|39S ribosomal protein L31, mitochondrial|mitochondrial large ribosomal subunit protein mL42

For more info on MRPL42, check out the MRPL42 Infographic

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In this infographic, you will see the following information for MRPL42: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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