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Product Info Summary
| SKU: | A01610-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-TPX2 Antibody Picoband®
SKU/Catalog Number
A01610-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TPX2 Antibody Picoband® catalog # A01610-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TPX2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01610-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human TPX2 recombinant protein (Position: D15-Q93).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01610-1 is reactive to TPX2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
86 kDa, 100 kDa
Calculated molecular weight
85.653kDa
Background of TPX2
Targeting protein for Xklp2, also known as C20ORF1, is a protein that in humans is encoded by the TPX2 gene. It is mapped to chromosome 20q11.2. It was concluded that even in the presence of duplicated centrosomes, spindle formation requires the function of TPX2 to generate a stable bipolar spindle with overlapping antiparallel microtubule arrays. At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01610-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human Hela whole cell, human COLO-320 whole cell, human U-87MG whole cell, human SGC-7901 whole cell, rat PC-12 whole cell, mouse HEPA1-6 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TPX2 using anti-TPX2 antibody (A01610-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human COLO-320 whole cell lysates,
Lane 3: human U-87MG whole cell lysates,
Lane 4: human SGC-7901 whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: mouse HEPA1-6 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TPX2 antigen affinity purified polyclonal antibody (Catalog # A01610-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TPX2 at approximately 86KD, 100KD. The expected band size for TPX2 is at 86KD.
Protein Target Info & Infographic
Gene/Protein Information For TPX2 (Source: Uniprot.org, NCBI)
Gene Name
TPX2
Full Name
Targeting protein for Xklp2
Weight
85.653kDa
Superfamily
TPX2 family
Alternative Names
Targeting protein for Xklp2; Differentially expressed in cancerous and non-cancerous lung cells 2; DIL-2; Hepatocellular carcinoma-associated antigen 519; Hepatocellular carcinoma-associated antigen 90; Protein fls353; Restricted expression proliferation-associated protein 100; p100; TPX2; C20orf1; C20orf2; DIL2; HCA519 TPX2 C20orf1, C20orf2, DIL-2, DIL2, FLS353, GD:C20orf1, HCA519, HCTP4, REPP86, p100 TPX2 microtubule nucleation factor targeting protein for Xklp2|TPX2, microtubule-associated protein homolog|TPX2, microtubule-associated, homolog|differentially expressed in cancerous and non-cancerous lung cells 2|differentially expressed in lung cells|hepatocellular carcinoma-associated 519|hepatocellular carcinoma-associated 90|preferentially expressed in colorectal cancer|protein fls353|restricted expression proliferation associated protein 100
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TPX2, check out the TPX2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TPX2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TPX2 Antibody Picoband® (A01610-1)
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Customer Q&As
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3 Customer Q&As for Anti-TPX2 Antibody Picoband®
Question
I was wanting to use your anti-TPX2 antibody for ELISA for human brain lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain lung identification?
Verified Customer
Verified customer
Asked: 2019-02-06
Answer
It shows on the product datasheet, A01610-1 anti-TPX2 antibody has been tested for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-06
Question
Will anti-TPX2 antibody A01610-1 work on pig ELISA with brain lung?
C. Moore
Verified customer
Asked: 2018-10-09
Answer
Our lab technicians have not tested anti-TPX2 antibody A01610-1 on pig. You can run a BLAST between pig and the immunogen sequence of anti-TPX2 antibody A01610-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig brain lung in ELISA, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-09
Question
We are currently using anti-TPX2 antibody A01610-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2017-11-13
Answer
The anti-TPX2 antibody (A01610-1) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-13
