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Product Info Summary
| SKU: | M01118 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Monkey |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-TORC2 CRTC2 Monoclonal Antibody
SKU/Catalog Number
M01118
Size
100ul
Form
Liquid
Description
Boster Bio Anti-TORC2 CRTC2 Monoclonal Antibody catalog # M01118. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Monkey.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TORC2 CRTC2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01118)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
5B10
Isotype
IgG
Immunogen
Purified recombinant fragment of human TORC2 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M01118 is reactive to CRTC2 in Human, Monkey
Reconstitution
Calculated molecular weight
73302 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01118 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Validation Images & Assay Conditions
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Figure 4. Flow Cytometry validation of CRTC2 using Anti-TORC2 CRTC2 Monoclonal Antibody (M01118).
Flow cytometric (FCM) analysis of HeLa cells using TORC2 Monoclonal Antibody (green) and negative control (purple).
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Figure 3. Immunofluorescent staining data of CRTC2 using Anti-TORC2 CRTC2 Monoclonal Antibody (M01118).
Immunofluorescence (IF) analysis of HeLa cells using TORC2 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Figure 2. Immunohistochemistry validation of CRTC2 using Anti-TORC2 CRTC2 Monoclonal Antibody (M01118).
Immunohistochemistry (IHC) analysis of paraffin-embedded ovary tumour tissues (left) and lung cancer (right) with DAB staining using TORC2 Monoclonal Antibody.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-TORC2 CRTC2 Monoclonal Antibody M01118
Western Blot (WB) analysis using TORC2 Monoclonal Antibody against HeLa (1) and HEK293 (2) cell lysate.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For CRTC2 (Source: Uniprot.org, NCBI)
Gene Name
CRTC2
Full Name
CREB-regulated transcription coactivator 2
Weight
73302 MW
Superfamily
TORC family
Alternative Names
CREB-regulated transcription coactivator 2;Transducer of regulated cAMP response element-binding protein 2;TORC-2;Transducer of CREB protein 2;CRTC2;TORC2; Crtc2|4632407F12Rik, Torc2|CREB regulated transcription coactivator 2|CREB-regulated transcription coactivator 2|TORC-2|transducer of CREB protein 2|transducer of regulated cAMP response element-binding protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CRTC2, check out the CRTC2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CRTC2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TORC2 CRTC2 Monoclonal Antibody (M01118)
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No publications found for M01118
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