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Product Info Summary
| SKU: | FEK0596 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and urine |
| Assay Time: | 90 Minutes |
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Product info
Introduction
Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.
Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol
Product Name
Mouse Adiponectin PicoKine® Quick ELISA Kit
See all Adiponectin/Acrp30 products
SKU/Catalog Number
FEK0596
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Adiponectin PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Adipoq in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Mouse Adiponectin PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0596)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: E18-N244
Sensitivity
<10 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK0596 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0596 is reactive to Adipoq in Mouse samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and urine
Application Guarantee
FEK0596 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Adiponectin/Acrp30
Adiponectin (ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK0596-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK0596-ST | Mouse Adiponectin/Acrp30 Standard | 2 | 20 ng/tube |
| FEK0596-A | Mouse Adipoq Antibody A | 1 | 3ml |
| FEK0596-B | Mouse Adipoq Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.021 | 0.068 | 0.126 | 0.393 | 0.623 | 1.039 | 1.452 | 2.063 |
Data Example Images
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Mouse Adiponectin PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Adiponectin PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 567 | 2994 | 7572 | 512 | 3121 | 7518 |
| Standard deviation | 37.98 | 179.64 | 318.02 | 37.75 | 240.31 | 330.79 |
| CV (%) | 6.7% | 6% | 4.2% | 7.1% | 7.7% | 4.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Adiponectin/Acrp30 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 567 | 603 | 628 | 571 | 592 | 24.91 | 4.2% |
| Sample 2 | 2994 | 2867 | 2845 | 2816 | 2880 | 67.97 | 2.3% |
| Sample 3 | 7572 | 7510 | 8167 | 7413 | 7665 | 295.03 | 3.8% |
Protein Target Info & Infographic
Gene/Protein Information For Adipoq (Source: Uniprot.Org, NCBI)
Gene Name
Adipoq
Full Name
Adiponectin
Weight
26809 MW
Alternative Names
Adiponectin;30 kDa adipocyte complement-related protein;Adipocyte complement-related 30 kDa protein;ACRP30;Adipocyte, C1q and collagen domain-containing protein;Adipocyte-specific protein AdipoQ;Adipoq;Acdc, Acrp30, Apm1; ADIPOQ ACDC, ACRP30, ADIPQTL1, ADPN, APM-1, APM1, GBP28 adiponectin, C1Q and collagen domain containing adiponectin|30 kDa adipocyte complement-related protein|adipocyte complement-related 30 kDa protein|adipose most abundant gene transcript 1 protein|adipose specific collagen-like factor|gelatin-binding protein 28
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Adipoq, check out the Adipoq Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Adipoq: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Adiponectin PicoKine® Quick ELISA Kit (FEK0596)
Hello CJ!
FEK0596 has been cited in 8 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Oral Administration of Resveratrol Alleviates Osteoarthritis Pathology in C57BL/6J Mice Model Induced by a High-Fat Diet
Puerarin Protects Pancreatic ?-Cells in Obese Diabetic Mice via Activation of GLP-1R Signaling
Apple Polyphenols Decrease Atherosclerosis and Hepatic Steatosis in ApoE?/? Mice through the ROS/MAPK/NF-?B Pathway
Dietary effects on liver tumor burden in mice treated with the hepatocellular carcinogen diethylnitrosamine
Chen, M., Wang, X., Hu, Z., Zhou, H., Xu, Y., Qiu, L.,…, & Ying, Z. (2017). Programming of mouse obesity by maternal exposure to concentrated ambient fine particles. Particle and Fibre Toxicology, 14(1), 20. doi: 10.1186/s12989-017-0201-9
Liu Xx, Liu Ky, Li P, Han S, Peng Xd, Shen L. Diabetes Metab. 2014 Nov;40(5):363-72. Doi: 10.1016/J.Diabet.2014.05.004. Epub 2014 Jun 27. Adiponectin Is Expressed In The Pancreas Of High-Fat-Diet-Fed Mice And Protects Pancreatic Endothelial Functi...
Liu L, Gu H, Zhao Y, An L, Yang J. Exp Ther Med. 2014 Dec;8(6):1813-1818. Epub 2014 Sep 30. Glypican 4 May Be Involved In The Adipose Tissue Redistribution In High-Fat Feeding C57Bl/6J Mice With Peroxisome Proliferators-Activated Receptor ?? Agoni...
Wu Cc, Weng Wl, Lai Wl, Tsai Hp, Liu Wh, Lee Mh, Tsai Yc. Evid Based Complement Alternat Med. 2015;2015:391767. Doi: 10.1155/2015/391767. Epub 2015 Feb 23. Effect Of Lactobacillus Plantarum Strain K21 On High-Fat Diet-Fed Obese Mice.
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