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Product Info Summary
| SKU: | A02841-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MC3 Receptor/MC3R Antibody Picoband®
View all Melanocortin-3 R/MC3R Antibodies
SKU/Catalog Number
A02841-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MC3 Receptor/MC3R Antibody Picoband® catalog # A02841-2. Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MC3 Receptor/MC3R Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02841-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human MC3 Receptor, different from the related mouse sequence by six amino acids, and from the related rat sequence by seven amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02841-2 is reactive to MC3R in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
40 kDa
Calculated molecular weight
36043 MW
Background of Melanocortin-3 R/MC3R
Melanocortin receptor 3 is a protein that in humans is encoded by the MC3R gene. It is mapped to 20q13.2. This gene encodes a G-protein-coupled receptor for melanocyte-stimulating hormone and adrenocorticotropic hormone that is expressed in tissues other than the adrenal cortex and melanocytes. This gene maps to the same region as the locus for benign neonatal epilepsy. Mice deficient for this gene have increased fat mass despite decreased food intake, suggesting a role for this gene product in the regulation of energy homeostasis. Mutations in this gene are associated with a susceptibility to obesity in humans.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02841-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse
Positive Control
WB: mouse kidney tissue, COLO320 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MC3 Receptor using anti-MC3 Receptor antibody (A02841-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
lane 1: mouse kidney tissue lysate,
lane 2: COLO320 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MC3 Receptor antigen affinity purified polyclonal antibody (Catalog # A02841-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MC3 Receptor at approximately 40KD. The expected band size for MC3 Receptor is at 36KD.
Protein Target Info & Infographic
Gene/Protein Information For MC3R (Source: Uniprot.org, NCBI)
Gene Name
MC3R
Full Name
Melanocortin receptor 3
Weight
36043 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Melanocortin receptor 3;MC3-R;MC3R; MC3R BMIQ9, MC3, MC3-R, OB20, OQTL melanocortin 3 receptor melanocortin receptor 3|obesity quantitative trait locus
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MC3R, check out the MC3R Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MC3R: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MC3 Receptor/MC3R Antibody Picoband® (A02841-2)
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No publications found for A02841-2
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Customer Q&As
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1 Customer Q&As for Anti-MC3 Receptor/MC3R Antibody Picoband®
Question
We are currently using anti-MC3 Receptor/MC3R antibody A02841-2 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
The anti-MC3 Receptor/MC3R antibody (A02841-2) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-22
