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Product Info Summary
| SKU: | A06631-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-TOR1AIP1 Antibody Picoband®
SKU/Catalog Number
A06631-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TOR1AIP1 Antibody Picoband® catalog # A06631-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TOR1AIP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06631-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TOR1AIP1 recombinant protein (Position: M1-D445).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06631-1 is reactive to TOR1AIP1 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
66 kDa
Calculated molecular weight
104553 MW
Background of TOR1AIP1
Torsin-1A-interacting protein 1 is a protein that in humans is encoded by the TOR1AIP1 gene. This gene encodes a type 2 integral membrane protein that binds A- and B-type lamins. The encoded protein localizes to the inner nuclear membrane and may be involved in maintaining the attachment of the nuclear membrane to the nuclear lamina during cell division. Alternate splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06631-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human MCF-7 whole cell, human Hela whole cell
ICC/IF: SiHa cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TOR1AIP1 using anti-TOR1AIP1 antibody (A06631-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOR1AIP1 antigen affinity purified polyclonal antibody (Catalog # A06631-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOR1AIP1 at approximately 66 kDa. The expected band size for TOR1AIP1 is at 66 kDa.
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Figure 2. IF analysis of TOR1AIP1 using anti-TOR1AIP1 antibody (A06631-1).
TOR1AIP1 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TOR1AIP1 Antibody (A06631-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The tissue section was developed using Phalloidin-iFluor 488 Conjugated. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For TOR1AIP1 (Source: Uniprot.org, NCBI)
Gene Name
TOR1AIP1
Full Name
Torsin-1A-interacting protein 1
Weight
104553 MW
Superfamily
TOR1AIP family
Alternative Names
Pancreatic secretory granule membrane major glycoprotein GP2; Pancreatic zymogen granule membrane protein GP-2; Gp2 TOR1AIP1 LAP1, LAP1B, LAP1C, LGMD2Y torsin 1A interacting protein 1 torsin-1A-interacting protein 1|lamin-associated protein 1B|lamina-associated polypeptide 1B|torsin A interacting protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TOR1AIP1, check out the TOR1AIP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TOR1AIP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TOR1AIP1 Antibody Picoband® (A06631-1)
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