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Product Info Summary
| SKU: | A00812-Dyl550 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human CD163 DyLight® 550 conjugated Antibody
SKU/Catalog Number
A00812-Dyl550
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human CD163 DyLight® 550 conjugated Antibody catalog # A00812-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human CD163 DyLight® 550 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00812-Dyl550)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CD163 recombinant protein (Position: T47-E201).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00812-Dyl550 is reactive to CD163 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
125.451kDa
Background of CD163
CD163 (Cluster of Differentiation 163) is a protein that in humans is encoded by the CD163 gene. The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00812-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Validation Images & Assay Conditions
Click image to see more details
Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For CD163 (Source: Uniprot.org, NCBI)
Gene Name
CD163
Full Name
Scavenger receptor cysteine-rich type 1 protein M130
Weight
125.451kDa
Alternative Names
Scavenger receptor cysteine-rich type 1 protein M130; Hemoglobin scavenger receptor; CD163; Soluble CD163; sCD163; CD163; M130 CD163 M130, MM130, SCARI1 CD163 molecule scavenger receptor cysteine-rich type 1 protein M130|hemoglobin scavenger receptor|macrophage-associated
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD163, check out the CD163 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD163: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human CD163 DyLight® 550 conjugated Antibody (A00812-Dyl550)
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-Human CD163 DyLight® 550 conjugated Antibody
Question
Our team were content with the WB result of your anti-Human CD163 DyLight® 550 conjugated antibody. However we have seen positive staining in spleen soluble cd163: secreted using this antibody. Is that expected? Could you tell me where is CD163 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
Based on literature, spleen does express CD163. Generally CD163 expresses in soluble cd163: secreted, cell membrane. Regarding which tissues have CD163 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218, 24275569
Plasma, Pubmed ID: 16335952
Spleen, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-04-01
Question
I see that the anti-Human CD163 DyLight® 550 conjugated antibody A00812-Dyl550 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-16
Question
Is this A00812-Dyl550 anti-Human CD163 DyLight® 550 conjugated antibody reactive to the isotypes of CD163?
C. Parker
Verified customer
Asked: 2019-11-29
Answer
The immunogen of A00812-Dyl550 anti-Human CD163 DyLight® 550 conjugated antibody is E. coli-derived human CD163 recombinant protein (Position: T47-E201). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-29
Question
Do you have a BSA free version of anti-Human CD163 DyLight® 550 conjugated antibody A00812-Dyl550 available?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Human CD163 DyLight® 550 conjugated antibody A00812-Dyl550 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-14
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for esophagus using anti-Human CD163 DyLight® 550 conjugated antibody A00812-Dyl550. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-24
Question
We are currently using anti-Human CD163 DyLight® 550 conjugated antibody A00812-Dyl550 for human tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
The anti-Human CD163 DyLight® 550 conjugated antibody (A00812-Dyl550) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-15
Question
Would anti-Human CD163 DyLight® 550 conjugated antibody A00812-Dyl550 work for Flow Cytometry with esophagus?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
According to the expression profile of esophagus, CD163 is highly expressed in esophagus. So, it is likely that anti-Human CD163 DyLight® 550 conjugated antibody A00812-Dyl550 will work for Flow Cytometry with esophagus.
Boster Scientific Support
Answered: 2019-09-25
Question
Is a blocking peptide available for product anti-Human CD163 DyLight® 550 conjugated antibody (A00812-Dyl550)?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
We do provide the blocking peptide for product anti-Human CD163 DyLight® 550 conjugated antibody (A00812-Dyl550). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-09
Question
I was wanting to use your anti-Human CD163 DyLight® 550 conjugated antibody for Flow Cytometry for human esophagus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human esophagus identification?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
It shows on the product datasheet, A00812-Dyl550 anti-Human CD163 DyLight® 550 conjugated antibody has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human esophagus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-19
Question
Our lab want to know about to test anti-Human CD163 DyLight® 550 conjugated antibody A00812-Dyl550 on human esophagus for research purposes, then I may be interested in using anti-Human CD163 DyLight® 550 conjugated antibody A00812-Dyl550 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
The products we sell, including anti-Human CD163 DyLight® 550 conjugated antibody A00812-Dyl550, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-09
Question
Please see the WB image, lot number and protocol we used for esophagus using anti-Human CD163 DyLight® 550 conjugated antibody A00812-Dyl550. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-02-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-04
Question
My question regarding product A00812-Dyl550, anti-Human CD163 DyLight® 550 conjugated antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-11-21
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00812-Dyl550 anti-Human CD163 DyLight® 550 conjugated antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-11-21
Question
Does A00812-Dyl550 anti-Human CD163 DyLight® 550 conjugated antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-09-18
Answer
As indicated on the product datasheet, A00812-Dyl550 anti-Human CD163 DyLight® 550 conjugated antibody as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-09-18
Question
We have observed staining in human esophagus. What should we do? Is anti-Human CD163 DyLight® 550 conjugated antibody supposed to stain esophagus positively?
N. Parker
Verified customer
Asked: 2013-11-01
Answer
Based on literature esophagus does express CD163. Based on Uniprot.org, CD163 is expressed in esophagus, spleen, plasma, liver, among other tissues. Regarding which tissues have CD163 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218, 24275569
Plasma, Pubmed ID: 16335952
Spleen, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2013-11-01
