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Product Info Summary
| SKU: | A02009 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-TNF12 TNFSF12 Antibody
View all TWEAK/TNFSF12 Antibodies
SKU/Catalog Number
A02009
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-TNF12 TNFSF12 Antibody (Catalog # A02009). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TNF12 TNFSF12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02009)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human TNF12.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02009 is reactive to TNFSF12 in Human, Mouse
Reconstitution
Calculated molecular weight
27216 MW
Background of TWEAK/TNFSF12
Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion.
Chicheportiche Y., J. Biol. Chem. 272:32401-32410(1997).
Marsters S.A., Curr. Biol. 8:525-528(1998).
Clark H.F., Genome Res. 13:2265-2270(2003).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02009 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human brain tissue using TNF12 antibody A02009.
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Western blot analysis of extracts from COLO cells, HUVEC cells and 293 cells, using TNF12 antibody A02009.
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Immunofluorescence analysis of HeLa cells, using TNF12 antibody A02009.
Protein Target Info & Infographic
Gene/Protein Information For TNFSF12 (Source: Uniprot.org, NCBI)
Gene Name
TNFSF12
Full Name
Tumor necrosis factor ligand superfamily member 12
Weight
27216 MW
Superfamily
tumor necrosis factor family
Alternative Names
Tumor necrosis factor ligand superfamily member 12;APO3 ligand;TNF-related weak inducer of apoptosis;TWEAK;Tumor necrosis factor ligand superfamily member 12, membrane form;Tumor necrosis factor ligand superfamily member 12, secreted form;TNFSF12;APO3L, DR3LG;UNQ181/PRO207; TNFSF12 APO3L, DR3LG, TNLG4A, TWEAK TNF superfamily member 12 tumor necrosis factor ligand superfamily member 12|APO3 ligand|APO3/DR3 ligand|TNF-related WEAK inducer of apoptosis|tumor necrosis factor (ligand) superfamily, member 12|tumor necrosis factor ligand 4A|tumor necrosis factor superfamily member 12
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TNFSF12, check out the TNFSF12 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TNFSF12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TNF12 TNFSF12 Antibody (A02009)
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No publications found for A02009
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-TNF12 TNFSF12 Antibody
Question
My question regarding product A02009, anti-TNF12 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02009 anti-TNF12 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-02
Question
Our lab want to know about to test anti-TNF12 antibody A02009 on mouse fetal kidney for research purposes, then I may be interested in using anti-TNF12 antibody A02009 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
R. Mitchell
Verified customer
Asked: 2019-10-15
Answer
The products we sell, including anti-TNF12 antibody A02009, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-15
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal kidney using anti-TNF12 antibody A02009. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-09-12
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-09-12
Question
Is there a BSA free version of anti-TNF12 antibody A02009 available?
J. Edwards
Verified customer
Asked: 2017-09-13
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TNF12 antibody A02009 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-09-13
Question
Would A02009 anti-TNF12 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
O. Williams
Verified customer
Asked: 2016-07-27
Answer
It shows on the product datasheet, A02009 anti-TNF12 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-07-27
Question
I was wanting to use your anti-TNF12 antibody for WB for mouse fetal kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse fetal kidney identification?
N. Jones
Verified customer
Asked: 2014-12-08
Answer
As indicated on the product datasheet, A02009 anti-TNF12 antibody has been validated for IF, IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse fetal kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-12-08
Question
Please see the WB image, lot number and protocol we used for fetal kidney using anti-TNF12 antibody A02009. Please let me know if you require anything else.
K. Jones
Verified customer
Asked: 2014-10-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-10-20
