Anti-Hu CD255 Purified TNFSF12 Monoclonal Antibody

Product Name

Anti-Hu CD255 Purified TNFSF12 Monoclonal Antibody

View all TWEAK/TNFSF12 Antibodies

SKU/Catalog Number

M02009

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD255 Purified TNFSF12 Monoclonal Antibody (Catalog# M02009). Tested in Flow Cytometry, IHC application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD255 Purified TNFSF12 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02009)

Host

Mouse

Contents

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

CARL-1

Isotype

Mouse IgG3 kappa

Immunogen

Human CD255-transfected 2PK-3 cells. The mouse monoclonal antibody CARL-1 recognizes an extracellular epitope of CD255 / TWEAK, a type II transmembrane protein of the TNF superfamily able to induce apoptosis weakly in many cell types.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody does not cross-react with Thy-1.1 alloantigen.

Reactive Species

M02009 is reactive to TNFSF12 in Human

Reconstitution

Observed Molecular Weight

42 kDa

Calculated molecular weight

27216 MW

Background of TWEAK/TNFSF12

CD255 / TWEAK (TNF-related weak inducer of apoptosis), a type II transmembrane protein expressed as membrane-bound and secreted form, can induce apoptosis in many tissues and cell lines through its receptor CD266 / TWEAK R. On the other hand, in endothelial cells this interaction can induce proliferation and promote angiogenesis including neovascularization of tumours. CD255 can act in a juxtacrine manner to initiate cellular responses, and induces secretion of pro-inflammatory cytokines. Besides CD266, CD255 may also bind to DR3.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M02009 is guaranteed for Flow Cytometry, IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 1-4 μg/ml.

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

Gene/Protein Information For TNFSF12 (Source: Uniprot.org, NCBI)

Alternative Names

TNFSF12, APO3L, DR3LG, TWEAK, TNLG4A TNFSF12 APO3L, DR3LG, TNLG4A, TWEAK TNF superfamily member 12 tumor necrosis factor ligand superfamily member 12|APO3 ligand|APO3/DR3 ligand|TNF-related WEAK inducer of apoptosis|tumor necrosis factor (ligand) superfamily, member 12|tumor necrosis factor ligand 4A|tumor necrosis factor superfamily member 12

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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