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Product Info Summary
| SKU: | A17417-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-TMEM52B Antibody Picoband®
SKU/Catalog Number
A17417-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TMEM52B Antibody Picoband® catalog # A17417-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TMEM52B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A17417-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TMEM52B recombinant protein (Position: T22-N183).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A17417-1 is reactive to TMEM52B in Human, Monkey, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
24 kDa
Calculated molecular weight
24145 MW
Background of TMEM52B
TMEM52B (Transmembrane Protein 52B, also known as C12orf59) is a Protein Coding gene. The TMEM52B gene, located on 12p13.2, is conserved in chimpanzee, dog, cow, mouse, rat, chicken, and frog. 2 alternatively spliced human isoforms have been reported. The 183-amino acid protein has a reported mass of 20,002 daltons. The cellular localization is predicted to be membrane-associated. C12orf59 might act as a potential oncogenic protein in gastric cancer (GC). Researchers propose that the decreased C12orf59 expression status is a prognostic biomarker of ccRCC and cooperates with the loss of von Hippel-Lindau (VHL) all the while promoting renal carcinogenesis. 192 organisms have orthologs with human gene TMEM52B.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A17417-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Monkey, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human 293T whole cell, monkey COS-7 whole cell, human SiHa whole cell, rat kidney tissue, rat NRK whole cell, mouse kidney tissue, mouse HBZY-1 whole cell
FCM: RT4 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TMEM52B using anti-TMEM52B antibody (A17417-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: monkey COS-7 whole cell lysates,
Lane 3: human SiHa whole cell lysates,
Lane 4: rat kidney tissue lysates,
Lane 5: rat NRK whole cell lysates,
Lane 6: mouse kidney tissue lysates,
Lane 7: mouse HBZY-1 whole cell lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM52B antigen affinity purified polyclonal antibody (Catalog # A17417-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM52B at approximately 24 kDa. The expected band size for TMEM52B is at 20 kDa.
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Figure 2. Flow Cytometry analysis of RT4 cells using anti-TMEM52B antibody (A17417-1).
Overlay histogram showing RT4 cells stained with A17417-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TMEM52B Antibody (A17417-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For TMEM52B (Source: Uniprot.org, NCBI)
Gene Name
TMEM52B
Full Name
Transmembrane protein 52B
Weight
24145 MW
Alternative Names
Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 1; PYROXD1 TMEM52B C12orf59 transmembrane protein 52B transmembrane protein 52B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TMEM52B, check out the TMEM52B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TMEM52B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TMEM52B Antibody Picoband® (A17417-1)
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