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Product Info Summary
| SKU: | PA1385 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Metalloproteinase inhibitor 1 TIMP1 Antibody Picoband®
SKU/Catalog Number
PA1385
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Metalloproteinase inhibitor 1 TIMP1 Antibody catalog # PA1385. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Metalloproteinase inhibitor 1 TIMP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1385)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human TIMP-1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1385 is reactive to TIMP1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
23 kDa
Calculated molecular weight
23171 MW
Background of TIMP-1
TIMP metallopeptidase inhibitor 1, also known as TIMP1, a tissue inhibitor of metalloproteinases, is a glycoprotein that is expressed from the several tissues of organisms. This protein a member of the TIMP family. The glycoprotein is a natural inhibitor of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. TIMP was found to be located about 22 cM proximal to OTC (300461).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1385 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human U-87MG whole cell, human U2OS whole cell, human PC-3 whole cell
IHC: Human Mammary Cancer Tissue
Validation Images & Assay Conditions
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Anti-TIMP-1 antibody, PA1385, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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Figure 1. Western blot analysis of TIMP1 using anti-TIMP1 antibody (PA1385).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates
Lane 2: human U2OS whole cell lysates
Lane 3: human PC-3 whole cell lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIMP1 antigen affinity purified polyclonal antibody (Catalog # PA1385) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIMP1 at approximately 23KD. The expected band size for TIMP1 is at 23KD.
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Figure 3. Western blot analysis of metalloproteinase inhibitor 1 TIMP1 using anti-Metalloproteinase inhibitor 1 TIMP1 antibody (PA1385).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% milk in TBST for 1.5 hour at RT. The membrane was incubated with rabbit anti-Metalloproteinase inhibitor 1 TIMP1 antibody (PA1385) at 1:250 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:2000 for 2 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for metalloproteinase inhibitor 1 TIMP1 at approximately 28 kDa. The expected band size for metalloproteinase inhibitor 1 TIMP1 is at 23 kDa.
Protein Target Info & Infographic
Gene/Protein Information For TIMP1 (Source: Uniprot.org, NCBI)
Gene Name
TIMP1
Full Name
Metalloproteinase inhibitor 1
Weight
23171 MW
Superfamily
protease inhibitor I35 (TIMP) family
Alternative Names
Metalloproteinase inhibitor 1;Erythroid-potentiating activity;EPA;Fibroblast collagenase inhibitor;Collagenase inhibitor;Tissue inhibitor of metalloproteinases 1;TIMP-1;TIMP1;CLGI, TIMP; TIMP1 CLGI, EPA, EPO, HCI, TIMP, TIMP-1 TIMP metallopeptidase inhibitor 1 metalloproteinase inhibitor 1|collagenase inhibitor|epididymis secretory sperm binding protein|erythroid potentiating activity|fibroblast collagenase inhibitor|tissue inhibitor of metalloproteinases 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TIMP1, check out the TIMP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TIMP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Metalloproteinase inhibitor 1 TIMP1 Antibody Picoband® (PA1385)
Hello CJ!
PA1385 has been cited in 23 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xu T,Pan L,Li L,Hu S,Zhou H,Yang C,Yang J,Li H,Liu Y,Meng X,Li J.MicroRNA-708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88. J Cell Mol Med. 2020 Jul;24(13):7127-7140.doi: 10.1111/jc
Species: Human
PA1385 usage in article: APP:WB, SAMPLE:LX-2 CELLS, DILUTION:1:1000
Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction
Effects of augmentation of liver regeneration recombinant plasmid on rat hepatic fibrosis
Recombinant human endostatin reduces hypertrophic scar formation in rabbit ear model through down-regulation of VEGF and TIMP-1
Matrix metalloproteinase-9 and vascular endothelial growth factor expression change in experimental retinal neovascularization
MMP-1/2 and TIMP-1/2 expression levels, and the levels of collagenous and elastic fibers correlate with disease progression in a hamster model of tongue cancer
Andrographolide plays an important role in bleomycin-induced pulmonary fibrosis treatment
Expression of matrix metalloproteinases-8 and -9 and their tissue inhibitor in the condyles of diabetic rats with mandibular advancement
Sanmiao formula inhibits chondrocyte apoptosis and cartilage matrix degradation in a rat model of osteoarthritis
Effect of Danshao Huaxian capsule on expression of matrix metalloproteinase-1 and tissue inhibitor of metalloproteinase-1 in fibrotic liver of rats
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Customer Reviews
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1 Reviews For Anti-Metalloproteinase inhibitor 1 TIMP1 Antibody Picoband®
0
Good TIMP-1 Antibody for Western Blotting--Swati Naphade, Aging, Buck Institute, Postdoctoral Fellow
Excellent
Source: Biocompare.com
| Application | Western Blot |
|---|---|
| Sample | Human NSC cell lysates |
| Detection | ECL chemiluminescence |
"We study neurodegeneration in our lab and I was looking for an antibody for human TIMP-1. The antibody detects a band at 28kD, which is the predicted molecular weight of TIMP-1. Good antibody for TIMP-1, cleaner than other antibodies I have tested. In summary, this antibody is good for human samples and I highly recommend it."
Swati Naphade
Verified customer
Submitted 2019-02-15
Customer Q&As
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6 Customer Q&As for Anti-Metalloproteinase inhibitor 1 TIMP1 Antibody Picoband®
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for ovary using anti-TIMP1 antibody PA1385. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-05-04
Question
Will anti-TIMP1 antibody PA1385 work for WB with ovary?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
According to the expression profile of ovary, TIMP1 is highly expressed in ovary. So, it is likely that anti-TIMP1 antibody PA1385 will work for WB with ovary.
Boster Scientific Support
Answered: 2019-09-16
Question
I was wanting to use your anti-TIMP1 antibody for WB for human ovary on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human ovary identification?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
You can see on the product datasheet, PA1385 anti-TIMP1 antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human ovary in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-10
Question
Is this PA1385 anti-TIMP1 antibody reactive to the isotypes of TIMP1?
Verified Customer
Verified customer
Asked: 2019-02-21
Answer
The immunogen of PA1385 anti-TIMP1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human TIMP-1(189-207aa CLPREPGLCTWQSLRSQIA). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-02-21
Question
My question regards to test anti-TIMP1 antibody PA1385 on human ovary for research purposes, then I may be interested in using anti-TIMP1 antibody PA1385 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-07-17
Answer
The products we sell, including anti-TIMP1 antibody PA1385, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-07-17
Question
We are currently using anti-TIMP1 antibody PA1385 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
F. Brown
Verified customer
Asked: 2014-03-28
Answer
The anti-TIMP1 antibody (PA1385) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-03-28
