Product Info Summary
SKU: | A10501-3 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-TMBIM1 Antibody Picoband™
SKU/Catalog Number
A10501-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TMBIM1 Antibody Picoband™ catalog # A10501-3. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TMBIM1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10501-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human TMBIM1, which shares 87.5% and 93.8% amino acid (aa) sequence identity with mouse and rat TMBIM1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A10501-3 is reactive to TMBIM1 in Human, Mouse, Rat
Applications
A10501-3 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
35 kDa
Calculated molecular weight
41409 MW
Background of TMBIM1
Transmembrane BAX inhibitor motif-containing protein 1 is a protein that in humans is encoded by the TMBIM1 gene. TMBIM1 is a member of the 7 TMS (7 transmembrane domains) family of receptors known to mediate the activation of various transcription factors. TMBIM1 is identified as a novel modulator of NF-kB activation. A drosophila homolog of this protein (dNMDA1 with 40% homology to hTMBIM1) is reported to be unregulated during aging and oxidative stress.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
![a10501 3 tmbim1 primary antibodies wb testing 1 a10501 3 tmbim1 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/1/a10501-3-tmbim1-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of TMBIM1 using anti-TMBIM1 antibody (A10501-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human CACO-2 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse kidney tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMBIM1 antigen affinity purified polyclonal antibody (Catalog # A10501-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMBIM1 at approximately 35KD. The expected band size for TMBIM1 is at 35KD.
![a10501 3 tmbim1 primary antibodies ihc testing 2 a10501 3 tmbim1 primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/1/a10501-3-tmbim1-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of TMBIM1 using anti-TMBIM1 antibody (A10501-3).
TMBIM1 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-TMBIM1 Antibody (A10501-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a10501 3 tmbim1 primary antibodies ihc testing 3 a10501 3 tmbim1 primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/1/a10501-3-tmbim1-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of TMBIM1 using anti-TMBIM1 antibody (A10501-3).
TMBIM1 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-TMBIM1 Antibody (A10501-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a10501 3 tmbim1 primary antibodies ihc testing 4 a10501 3 tmbim1 primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/a/1/a10501-3-tmbim1-primary-antibodies-ihc-testing-4.jpg)
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Figure 4. IHC analysis of TMBIM1 using anti-TMBIM1 antibody (A10501-3).
TMBIM1 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-TMBIM1 Antibody (A10501-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a10501 3 tmbim1 primary antibodies fcm testing 5 a10501 3 tmbim1 primary antibodies fcm testing 5](https://www.bosterbio.com/media/catalog/product/a/1/a10501-3-tmbim1-primary-antibodies-fcm-testing-5.png)
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Figure 5. Flow Cytometry analysis of HepG2 cells using anti-TMBIM1 antibody (A10501-3).
Overlay histogram showing HepG2 cells stained with A10501-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMBIM1 Antibody (A10501-3, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For TMBIM1 (Source: Uniprot.org, NCBI)
Gene Name
TMBIM1
Full Name
Protein lifeguard 3
Weight
41409 MW
Superfamily
BI1 family
Alternative Names
LFG3; MST100; PP1201; Protein RECS1 homolog; RECS1MSTP100; transmembrane BAX inhibitor motif containing 1; transmembrane BAX inhibitor motif-containing protein 1 TMBIM1 LFG3, MST100, MSTP100, PP1201, RECS1 transmembrane BAX inhibitor motif containing 1 protein lifeguard 3|transmembrane BAX inhibitor motif-containing protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TMBIM1, check out the TMBIM1 Infographic
![TMBIM1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TMBIM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TMBIM1 Antibody Picoband™ (A10501-3)
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