Product Info Summary
SKU: | A06937-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-Semaphorin 3E/SEMA3E Antibody Picoband®
View all Semaphorin 3E Antibodies
SKU/Catalog Number
A06937-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Semaphorin 3E/SEMA3E Antibody Picoband® catalog # A06937-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Semaphorin 3E/SEMA3E Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06937-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Semaphorin 3E/SEMA3E recombinant protein (Position: F55-K677).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06937-2 is reactive to SEMA3E in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
89 kDa
Calculated molecular weight
87148 MW
Background of Semaphorin 3E
Semaphorin 3E is a protein that in humans is encoded by the SEMA3E gene. It is mapped to 7q21.11. Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. This gene encodes a class 4 semaphorin. This gene encodes a class 3 semaphorin. Multiple transcript variants encoding different isoforms have been found for this gene
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06937-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human U2OS whole cell, human U-87MG whole cell, rat testicular tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SEMA3E using anti-SEMA3E antibody (A06937-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U2OS whole cell lysates,
Lane 2: human U-87MG whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEMA3E antigen affinity purified polyclonal antibody (Catalog # A06937-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEMA3E at approximately 89KD. The expected band size for SEMA3E is at 89KD.
Click image to see more details
Figure 2. Western blot analysis of SEMA3E using anti-SEMA3E antibody (A06937-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat testicular tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEMA3E antigen affinity purified polyclonal antibody (Catalog # A06937-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEMA3E at approximately 89KD. The expected band size for SEMA3E is at 89KD.
Protein Target Info & Infographic
Gene/Protein Information For SEMA3E (Source: Uniprot.org, NCBI)
Gene Name
SEMA3E
Full Name
Semaphorin-3E
Weight
87148 MW
Superfamily
semaphorin family
Alternative Names
Semaphorin-3E; SEMA3E; KIAA0331; SEMAH SEMA3E M-SEMAH, M-SemaK, SEMAH, coll-5 semaphorin 3E semaphorin-3E|sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SEMA3E, check out the SEMA3E Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SEMA3E: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Semaphorin 3E/SEMA3E Antibody Picoband® (A06937-2)
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Customer Q&As
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4 Customer Q&As for Anti-Semaphorin 3E/SEMA3E Antibody Picoband®
Question
I see that the anti-Semaphorin 3E/SEMA3E antibody A06937-2 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-12
Question
Would anti-Semaphorin 3E/SEMA3E antibody A06937-2 work for WB with brain?
J. Jackson
Verified customer
Asked: 2017-10-27
Answer
According to the expression profile of brain, SEMA3E is highly expressed in brain. So, it is likely that anti-Semaphorin 3E/SEMA3E antibody A06937-2 will work for WB with brain.
Boster Scientific Support
Answered: 2017-10-27
Question
We are currently using anti-Semaphorin 3E/SEMA3E antibody A06937-2 for mouse tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
W. Johnson
Verified customer
Asked: 2017-02-20
Answer
The anti-Semaphorin 3E/SEMA3E antibody (A06937-2) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-02-20
Question
I was wanting to use your anti-Semaphorin 3E/SEMA3E antibody for WB for rat brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat brain identification?
J. Miller
Verified customer
Asked: 2016-11-24
Answer
As indicated on the product datasheet, A06937-2 anti-Semaphorin 3E/SEMA3E antibody has been tested for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-11-24