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Product Info Summary
| SKU: | PB9300 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Neuropilin 1/NRP1 Antibody Picoband®
View all Neuropilin-1 Antibodies
SKU/Catalog Number
PB9300
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Neuropilin 1/NRP1 Antibody Picoband® catalog # PB9300. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Neuropilin 1/NRP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9300)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Neuropilin 1 recombinant protein (Position: K504-T827). Human Neuropilin 1 shares 95% and 94% amino acid (aa) sequences identity with mouse and rat Neuropilin 1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9300 is reactive to NRP1 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
103 kDa
Calculated molecular weight
103134 MW
Background of Neuropilin-1
This gene encodes one of two neuropilins, which contain specific protein domains which allow them to participate in several different types of signaling pathways that control cell migration. Neuropilins contain a large N-terminal extracellular domain, made up of complement-binding, coagulation factor V/VIII, and meprin domains. These proteins also contain a short membrane-spanning domain and a small cytoplasmic domain. Neuropilins bind many ligands and various types of co-receptors; they affect cell survival, migration, and attraction. Some of the ligands and co-receptors bound by neuropilins are vascular endothelial growth factor (VEGF) and semaphorin family members. Several alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9300 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: U87 Whole Cell, A549 Whole Cell, Human Placenta Tissue, Rat Cardiac Muscle Tissue
IHC: Human Placenta Tissue
Validation Images & Assay Conditions
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Anti-Neuropillin-1 Picoband antibody, PB9300, Western blotting
All lanes: Anti Neuropillin-1 (PB9300) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: Human Placenta Tissue Lysate at 50ug
Lane 4: Rat Cardiac Muscle Tissue Lysate at 50ug
Predicted bind size: 103KD
Observed bind size: 103KD
Click image to see more details
Anti-Neuropillin-1 Picoband antibody, PB9300, IHC(P)
IHC(P): Human Placenta Tissue
Protein Target Info & Infographic
Gene/Protein Information For NRP1 (Source: Uniprot.org, NCBI)
Gene Name
NRP1
Full Name
Neuropilin-1
Weight
103134 MW
Superfamily
neuropilin family
Alternative Names
Neuropilin-1;Vascular endothelial cell growth factor 165 receptor;CD304;NRP1;NRP, VEGF165R; NRP1 BDCA4, CD304, NP1, NRP, VEGF165R neuropilin 1 neuropilin-1|transmembrane receptor|vascular endothelial cell growth factor 165 receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NRP1, check out the NRP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NRP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Neuropilin 1/NRP1 Antibody Picoband® (PB9300)
Hello CJ!
PB9300 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Dual Receptor Recognizing Cell Penetrating Peptide for Selective Targeting, Efficient Intratumoral Diffusion and Synthesized Anti-Glioma Therapy
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Neuropilin 1/NRP1 Antibody Picoband®
Question
We are interested in using your anti-Neuropilin 1/NRP1 antibody for semaphorin-plexin signaling pathway involved in neuron projection guidance studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
We appreciate your inquiry. This PB9300 anti-Neuropilin 1/NRP1 antibody is tested on u87 whole cell lysate, a549 whole cell lysate, human placenta tissue, tissue lysate, rat cardiac muscle tissue lysate. It is guaranteed to work for IHC, WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-25
Question
Do you have a BSA free version of anti-Neuropilin 1/NRP1 antibody PB9300 available?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Neuropilin 1/NRP1 antibody PB9300 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-04
Question
We ordered your anti-Neuropilin 1/NRP1 antibody for WB on prostatic adenocarcinoma a few months ago. I am using rat, and We want to use the antibody for IHC next. you antibody examining prostatic adenocarcinoma as well as caput epididymis in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
I took a look at the website and datasheets of our anti-Neuropilin 1/NRP1 antibody and it seems that PB9300 has been validated on rat in both WB and IHC. Thus PB9300 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-02-28
Question
Would PB9300 anti-Neuropilin 1/NRP1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
You can see on the product datasheet, PB9300 anti-Neuropilin 1/NRP1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-13
Question
I was wanting to use your anti-Neuropilin 1/NRP1 antibody for WB for human caput epididymis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human caput epididymis identification?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
You can see on the product datasheet, PB9300 anti-Neuropilin 1/NRP1 antibody has been validated for IHC, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human caput epididymis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-12
Question
Our team were satisfied with the WB result of your anti-Neuropilin 1/NRP1 antibody. However we have observed positive staining in plasma cell membrane using this antibody. Is that expected? Could you tell me where is NRP1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
From literature, plasma does express NRP1. Generally NRP1 expresses in cell membrane. Regarding which tissues have NRP1 expression, here are a few articles citing expression in various tissues:
Kidney, and Muscle, Pubmed ID: 15489334
Liver, Pubmed ID: 19159218, 24275569
Mammary gland, Pubmed ID: 9529250
Plasma, Pubmed ID: 16335952
Prostatic adenocarcinoma, Pubmed ID: 10688880
Skeletal muscle, Pubmed ID: 17974005
Boster Scientific Support
Answered: 2019-08-09
Question
Would anti-Neuropilin 1/NRP1 antibody PB9300 work for WB with caput epididymis?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
According to the expression profile of caput epididymis, NRP1 is highly expressed in caput epididymis. So, it is likely that anti-Neuropilin 1/NRP1 antibody PB9300 will work for WB with caput epididymis.
Boster Scientific Support
Answered: 2019-08-08
Question
We have observed staining in human kidney muscle. Do you have any suggestions? Is anti-Neuropilin 1/NRP1 antibody supposed to stain kidney muscle positively?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
From what I have seen in literature kidney muscle does express NRP1. From what I have seen in Uniprot.org, NRP1 is expressed in caput epididymis, mammary gland, prostatic adenocarcinoma, skeletal muscle, kidney muscle, plasma, liver, among other tissues. Regarding which tissues have NRP1 expression, here are a few articles citing expression in various tissues:
Kidney, and Muscle, Pubmed ID: 15489334
Liver, Pubmed ID: 19159218, 24275569
Mammary gland, Pubmed ID: 9529250
Plasma, Pubmed ID: 16335952
Prostatic adenocarcinoma, Pubmed ID: 10688880
Skeletal muscle, Pubmed ID: 17974005
Boster Scientific Support
Answered: 2019-06-11
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for caput epididymis using anti-Neuropilin 1/NRP1 antibody PB9300. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-03-15
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-03-15
Question
My lab would like to test anti-Neuropilin 1/NRP1 antibody PB9300 on human caput epididymis for research purposes, then I may be interested in using anti-Neuropilin 1/NRP1 antibody PB9300 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-03-15
Answer
The products we sell, including anti-Neuropilin 1/NRP1 antibody PB9300, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-03-15
Question
Please see the WB image, lot number and protocol we used for caput epididymis using anti-Neuropilin 1/NRP1 antibody PB9300. Please let me know if you require anything else.
A. Miller
Verified customer
Asked: 2018-01-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-16
Question
Is a blocking peptide available for product anti-Neuropilin 1/NRP1 antibody (PB9300)?
K. Dhar
Verified customer
Asked: 2017-12-14
Answer
We do provide the blocking peptide for product anti-Neuropilin 1/NRP1 antibody (PB9300). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-12-14
Question
We are currently using anti-Neuropilin 1/NRP1 antibody PB9300 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on goat tissues as well?
P. Brown
Verified customer
Asked: 2017-11-27
Answer
The anti-Neuropilin 1/NRP1 antibody (PB9300) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-27
Question
I see that the anti-Neuropilin 1/NRP1 antibody PB9300 works with WB, what is the protocol used to produce the result images on the product page?
B. Rodriguez
Verified customer
Asked: 2017-08-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-08-24
Question
Can you help my question with product PB9300, anti-Neuropilin 1/NRP1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-07-26
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9300 anti-Neuropilin 1/NRP1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-07-26
Question
Is this PB9300 anti-Neuropilin 1/NRP1 antibody reactive to the isotypes of NRP1?
D. Jones
Verified customer
Asked: 2017-04-11
Answer
The immunogen of PB9300 anti-Neuropilin 1/NRP1 antibody is E.coli-derived human Neuropilin 1 recombinant protein (Position: K504-T827). Human Neuropilin 1 shares 95% and 94% amino acid (aa) sequences identity with mouse and rat Neuropilin 1, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-04-11
