Anti-TM4SF1 Antibody

Product Name

Anti-TM4SF1 Antibody

View all TM4SF1/L6 Antibodies

SKU/Catalog Number

A10368

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-TM4SF1 Antibody (Catalog # A10368). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

TM4SF1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-TM4SF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10368)

Host

Rabbit

Contents

TM4SF1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

TM4SF1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human TM4SF1. The immunogen is located within the first 50 amino acids of TM4SF1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least three isoforms of TM4SF1 are known to exist; this antibody will recognize only the two largest isoforms. TM4SF1 antibody is predicted to not cross-react with other tetraspanin proteins.

Reactive Species

A10368 is reactive to TM4SF1 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

21632 MW

Background of TM4SF1/L6

TM4SF1 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains and mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. TM4SF1 is a cell surface antigen and is highly expressed in the vascular endothelium of several human cancers. Knockdown experiments of TM4SF1 prevented filopodia formation, inhibited cell mobility, blocked cytokinesis, and inhibited maturation of VEGF-A-induced angiogenesis, suggesting that TM4SF1 may be an attractive target for anti-angiogenesis therapy.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A10368 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

TM4SF1 antibody can be used for detection of TM4SF1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For TM4SF1 (Source: Uniprot.org, NCBI)

Alternative Names

L6, H-L6, M3S1, TAAL6, Transmembrane 4 L6 family member 1, Membrane component chromosome 3 surface marker 1 TM4SF1 H-L6, L6, M3S1, TAAL6 transmembrane 4 L six family member 1 transmembrane 4 L6 family member 1|membrane component, chromosome 3, surface marker 1|transmembrane 4 superfamily member 1|tumor-associated L6

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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