Key=store_id
Key=entity_id, value=47358
Key=attribute_set_id, value=4
Key=type_id, value=simple
Key=sku, value=EZ0903
Key=has_options, value=0
Key=required_options, value=0
Key=created_at, value=2018-04-28 12:24:09
Key=updated_at, value=2024-09-26 00:58:20
Key=mst_search_weight, value=0
Key=status, value=Enabled
Key=name, value=Human KLK1 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
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Key=template, value=ez-set
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Key=price, value=500.0000
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Key=meta_title, value=Human KLK1 ELISA Kit EZ-Set™ Pairs)
Key=image, value=/E/Z/EZ0903-human-klk1-ez-set-elisa-kit-diy-antibody-pairs.jpg
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Key=url_key, value=human-klk1-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0903-boster
Key=url_path, value=human-klk1-elisa-kit-ez-set-trade-diy-antibody-pairs
Key=options_container, value=Product Info Column
Key=image_label, value=Human KLK1 EZ-Set ELISA Kit standard curve
Key=small_image_label, value=Human KLK1 EZ-Set ELISA Kit standard curve
Key=thumbnail_label, value=Human KLK1 EZ-Set ELISA Kit standard curve
Key=msrp_display_actual_price_type, value=
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Key=size, value=For 5 plates, 96 wells each
Key=gene_full_name, value=kallikrein 1
Key=uniprot_id, value=P06870
Key=assay_range, value=156 pg/ml - 10,000 pg/ml
Key=applications, value=ELISA
Key=reactivity, value=Human
Key=cross_reactivity, value=There is no detectable cross-reactivity with other relevant proteins.
Key=anticoagulant, value=heparin or EDTA
Key=tmb_incubation_time, value=25-30
Key=source_company, value=Boster EZ0903
Key=product_category, value=EZ-Set ELISA Kits (Ab Pairs)
Key=research_category, value=Angiogenesis, Cancer, Cardiovascular, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Proteolysis/Ubiquitin, Proteolytic Enzymes, Serum Proteins, Signal Transduction
Key=swatch_image, value=/e/k/ek0903_1.png
Key=rating_value, value=97
Key=review_count, value=6
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Key=capture_antibody, value=Rabbit anti- human KLK1 polyclonal antibody
Key=detection_antibody, value=Biotinylated goat anti- human KLK1 polyclonal antibody
Key=cj_related_products, value=Seminal Clot Liquefaction:EK0816|PB9259|M01505-1|A01505-2|A01505-3|EK0903
Key=description, value=Human KLK1 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human KLK1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Key=short_description, value=Human KLK1 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human KLK1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Key=background, value=Kallikrein-1, also known as tissue kallikrein, is a protein that in humans is encoded by the KLK1 gene. This serine protease generates Lys-bradykinin by specific proteolysis of kininogen-1. KLK1 is a member of the peptidase S1 family. Its gene is mapped to 19q13.3. In all, it has got 262-amino acids which contain a putative signal peptide, followed by a short activating peptide and the protease domain. The protein is mainly found in kidney, pancreas, and salivary gland, showing a unique pattern of tissue-specific expression relative to other members of the family. KLK1 is implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.
Key=gene_name, value=KLK1
Key=synonyms, value=Kallikrein-1; Kidney/pancreas/salivary gland kallikrein; Tissue kallikrein; KLK1
Key=sample_type, value=cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Key=kit_components, value=

Catalog number	Description	Quantity	Storage of opened/reconstituted material
EZ0903-CA	Rabbit anti- human KLK1 polyclonal antibody (Capture Antibody)	500 µl, 0.4 mg/mL (recommended dilution 1:100)	Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
EZ0903-DA	Biotinylated goat anti- human KLK1 polyclonal antibody (Detection Antibody)	500 µl, 1 µg/mL (recommended dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	500 µl (recommended dilution 1:100)
EK0903-ST	Lyophilized recombinant human KLK1 standard	10 ng/tube×3	Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Key=intra_inter_assay_cv, value=

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	1468	3882	6352	1625	4190	6526
Standard deviation	67.53	260.1	349.4	95.9	301.7	417.7
CV (%)	4.6%	6.7%	5.5%	5.9%	7.2%	6.4%

Key=tissue_specificity, value= Isoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84.
Key=protein_function, value= Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
Key=sample_data, value=

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.016	0.084	0.157	0.322	0.513	0.965	1.516	2.02

Key=description_before_attributes, value=

Product Features:

Your ideal low cost ELISA solution. EZ Set™ ELISA kit contains the necessary core reagents develop and run the ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC). One kit is sufficient for developing 5 plates (96 well format). EZ Set™ ELISAs are ideal for experienced ELISA users on a budget and great for exploratory work--a fast and easy way to identify the relevant protein targets of interest/relevance to your studies.

Boster also provides the most comprehensive technical support materials and most user friendly technical support service to guarantee that you can do the experiment smoothly and successfully.

Key=componentsneeded, value=

1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate (Cat# AR1100)
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered.
10. Color Developing Reagent: Tetramethylbenzidine (Cat# AR1104)
11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
12. Wash Buffer (PBS and PBS-T).
  PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered.
  PBS-T: 0.1% Tween? 20 in PBS, pH 7.2-7.4.
*Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)

Key=immunogen, value=Expression system for standard: NS0; Immunogen sequence: I25-S262
Key=storage, value=Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Key=preparation_step_2, value=1) It is recommended that the standards be prepared no more than 2 hours prior to performing the experiment. Use one 10 ng of lyophilized human KLK1 standard for each experiment. Gently spin the vial prior to use. Reconstitute the standard to a stock concentration of 10 ng/ml using 1 ml of Reagent Diluent. Allow the standard to sit for a minimum of 10 minutes with gentle agitation prior to making dilutions.
2) Dilution of Human KLK1 Standard
• Number tubes 1 - 8. Final Concentrations to be Tube # 1 - 10,000 pg/ml, # 2 - 5,000 pg/ml, # 3 - 2,500 pg/ml, # 4 - 1,250 pg/ml, # 5 - 625 pg/ml, # 6 - 312 pg/ml, # 7 - 156 pg/ml, # 8 - 0.0 (Blank).
• For standard # 1, add 1,000 µl of undiluted standard stock solution to tube # 1.
• Add 300 µl of Reagent Diluent to tubes # 2 - 7.
• To generate standard # 2, add 300 µl of standard # 1 from tube # 1 to tube # 2 for a final volume of 600 µl. Mix thoroughly.
• To generate standard # 3, add 300 µl of standard # 2 from tube # 2 to tube # 3 for a final volume of 600 µl. Mix thoroughly.
• Continue the serial dilution for tube # 4 - 7.
• Tube # 8 is a blank standard to be used with every experiment.
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