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Product Info Summary
| SKU: | A04277-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-TIEG2 (F6) KLF11 Antibody
SKU/Catalog Number
A04277-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-TIEG2 (F6) KLF11 Antibody catalog # A04277-1. Tested in WB,IHC,IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TIEG2 (F6) KLF11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04277-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from Internal of human KLF11.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04277-1 is reactive to KLF11 in Human, Mouse, Rat
Applications
A04277-1 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
55139 MW
Background of KLF11
Transcription factor. Activates the epsilon- and gamma-globin gene promoters and, to a much lower degree, the beta-globin gene and represses promoters containing SP1-like binding inhibiting cell growth. Represses transcription of SMAD7 which enhances TGF-beta signaling. Induces apoptosis.
Cook T., J. Biol. Chem. 273:25929-25936(1998).
Asano H., Mol. Cell. Biol. 19:3571-3579(1999).
Neve B., Proc. Natl. Acad. Sci. U.S.A. 102:4807-4812(2005).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of KLF11 using Anti-TIEG2 (F6) KLF11 Antibody (A04277-1).
Immunohistochemistry (IHC) analyzes of TIEG2 (F6) pAb in paraffin-embedded human liver carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody
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Figure 1. Western blotting validation for Anti-TIEG2 (F6) KLF11 Antibody A04277-1
Western blot (WB) analysis of TIEG2 (F6) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysate(40ug)
Lane2:THP-1 whole cell lysate(40ug)
Lane3:SGC7901 whole cell lysate(40ug)
Lane4:H9C2 whole cell lysate(40ug)
Lane5:The Heart tissue lysate of Mouse(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For KLF11 (Source: Uniprot.org, NCBI)
Gene Name
KLF11
Full Name
Krueppel-like factor 11
Weight
55139 MW
Superfamily
Sp1 C2H2-type zinc-finger protein family
Alternative Names
FKLFFKLF1; Krueppel-like factor 11; Kruppel-like factor 11; MODY7TGFB-inducible early growth response protein 2; TIEG-2; TIEG2TGFB inducible early growth response 2; Tieg3; Transforming growth factor-beta-inducible early growth response protein 2 KLF11 FKLF, FKLF1, MODY7, TIEG2, Tieg3 Kruppel like factor 11 Krueppel-like factor 11|TGFB-inducible early growth response protein 2|TIEG-2|transforming growth factor-beta-inducible early growth response protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KLF11, check out the KLF11 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KLF11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TIEG2 (F6) KLF11 Antibody (A04277-1)
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