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Product Info Summary
| SKU: | A04296 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-WTAP Antibody
SKU/Catalog Number
A04296
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-WTAP Antibody (Catalog # A04296). Tested in WB, IHC, IF/ICC, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-WTAP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04296)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from C-terminal of human WTAP.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04296 is reactive to WTAP in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
44244 MW
Background of WTAP
Regulatory subunit of the WMM N6-methyltransferase complex, a multiprotein complex that mediates N6-methyladenosine (m6A) methylation of some adenosine residues of some mRNAs and plays a role in the efficiency of mRNA splicing and processing and mRNA stability. Required for accumulation of METTL3 and METTL14 to nuclear speckle. Acts as a mRNA splicing regulator. Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes.
Ortega A., J. Biol. Chem. 278:3040-3047(2003).
Nagase T., DNA Res. 2:37-43(1995).
Mungall A.J., Nature 425:805-811(2003).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04296 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from HUVEC cells, using WTAP antibody A04296.
Protein Target Info & Infographic
Gene/Protein Information For WTAP (Source: Uniprot.org, NCBI)
Gene Name
WTAP
Full Name
Pre-mRNA-splicing regulator WTAP
Weight
44244 MW
Superfamily
fl(2)d family
Alternative Names
Pre-mRNA-splicing regulator WTAP;Female-lethal (2)D homolog;hFL (2)D;WT1-associated protein;Wilms tumor 1-associating protein;WTAP;KIAA0105; WTAP Mum2 WT1 associated protein pre-mRNA-splicing regulator WTAP|PNAS-132|Wilms tumor 1 associated protein|Wilms tumour 1-associating protein|female-lethal(2)D homolog|hFL(2)D|putative pre-mRNA splicing regulator female-lethal(2D)|wilms tumor 1-associating protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on WTAP, check out the WTAP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for WTAP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-WTAP Antibody (A04296)
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No publications found for A04296
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Recommended Resources
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-WTAP Antibody
Question
I see that the anti-WTAP antibody A04296 works with WB, what is the protocol used to produce the result images on the product page?
E. Bhatt
Verified customer
Asked: 2020-02-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-24
Question
Is a blocking peptide available for product anti-WTAP antibody (A04296)?
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
We do provide the blocking peptide for product anti-WTAP antibody (A04296). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-11
Question
My question regarding product A04296, anti-WTAP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
E. Johnson
Verified customer
Asked: 2019-10-21
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04296 anti-WTAP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-21
Question
Is there a BSA free version of anti-WTAP antibody A04296 available?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-WTAP antibody A04296 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-23
Question
Does anti-WTAP antibody A04296 work on monkey WB with ovarian carcinoma?
J. Baker
Verified customer
Asked: 2019-05-06
Answer
Our lab technicians have not validated anti-WTAP antibody A04296 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-WTAP antibody A04296 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey ovarian carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-06
Question
I have attached the WB image, lot number and protocol we used for cervix carcinoma using anti-WTAP antibody A04296. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-06
Question
Will anti-WTAP antibody A04296 work for WB with cervix carcinoma?
M. Miller
Verified customer
Asked: 2019-01-28
Answer
According to the expression profile of cervix carcinoma, WTAP is highly expressed in cervix carcinoma. So, it is likely that anti-WTAP antibody A04296 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2019-01-28
Question
I was wanting to use your anti-WTAP antibody for WB for mouse cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse cervix carcinoma identification?
S. Yang
Verified customer
Asked: 2018-03-23
Answer
It shows on the product datasheet, A04296 anti-WTAP antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-23
Question
My lab would like to test anti-WTAP antibody A04296 on mouse cervix carcinoma for research purposes, then I may be interested in using anti-WTAP antibody A04296 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
T. Parker
Verified customer
Asked: 2017-08-11
Answer
The products we sell, including anti-WTAP antibody A04296, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-11
Question
Does A04296 anti-WTAP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
O. Krishna
Verified customer
Asked: 2017-07-04
Answer
It shows on the product datasheet, A04296 anti-WTAP antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-07-04
Question
Is this A04296 anti-WTAP antibody reactive to the isotypes of WTAP?
L. Krishna
Verified customer
Asked: 2016-09-28
Answer
The immunogen of A04296 anti-WTAP antibody is Synthesized peptide derived from C-terminal of human WTAP. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-09-28
Question
We are currently using anti-WTAP antibody A04296 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on feline tissues as well?
G. Johnson
Verified customer
Asked: 2014-02-06
Answer
The anti-WTAP antibody (A04296) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-02-06
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-WTAP antibody A04296. Let me know if you need anything else.
M. Jha
Verified customer
Asked: 2013-04-03
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-04-03
