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Product Info Summary
| SKU: | A06485-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Mad/MXD1 Antibody Picoband®
SKU/Catalog Number
A06485-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Mad/MXD1 Antibody Picoband® catalog # A06485-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Mad/MXD1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06485-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Mad recombinant protein (Position: M1-L221).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06485-1 is reactive to MXD1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
28 kDa
Calculated molecular weight
25.254kDa
Background of Mad
MAD protein is a protein that in humans is encoded by the MXD1 gene. This gene encodes a member of the MYC/MAX/MAD network of basic helix-loop-helix leucine zipper transcription factors. The MYC/MAX/MAD transcription factors mediate cellular proliferation, differentiation and apoptosis. The encoded protein antagonizes MYC-mediated transcriptional activation of target genes by competing for the binding partner MAX and recruiting repressor complexes containing histone deacetylases. Mutations in this gene may play a role in acute leukemia, and the encoded protein is a potential tumor suppressor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06485-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: rat testis tissue, mouse ovary tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Mad using anti-Mad antibody (A06485-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: mouse ovary tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mad antigen affinity purified polyclonal antibody (Catalog # A06485-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mad at approximately 28KD. The expected band size for Mad is at 25KD.
Protein Target Info & Infographic
Gene/Protein Information For MXD1 (Source: Uniprot.org, NCBI)
Gene Name
MXD1
Full Name
Max dimerization protein 1
Weight
25.254kDa
Alternative Names
Max dimerization protein 1; Max dimerizer 1; Protein MAD; MXD1; MAD MXD1 BHLHC58, MAD, MAD1 MAX dimerization protein 1 max dimerization protein 1|MAX-binding protein|antagonizer of myc transcriptional activity|max dimerizer 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MXD1, check out the MXD1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MXD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Mad/MXD1 Antibody Picoband® (A06485-1)
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Customer Q&As
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4 Customer Q&As for Anti-Mad/MXD1 Antibody Picoband®
Question
Is there a BSA free version of anti-Mad/MXD1 antibody A06485-1 available?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Mad/MXD1 antibody A06485-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-25
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-Mad/MXD1 antibody A06485-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-09
Question
Here is the WB image, lot number and protocol we used for lung using anti-Mad/MXD1 antibody A06485-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-24
Question
We are currently using anti-Mad/MXD1 antibody A06485-1 for rat tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
C. Lewis
Verified customer
Asked: 2013-03-08
Answer
The anti-Mad/MXD1 antibody (A06485-1) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-03-08
