Product Info Summary
SKU: | A06424 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IF, WB |
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Product info
Product Name
Anti-SIN3B Antibody
SKU/Catalog Number
A06424
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-SIN3B Antibody (Catalog # A06424). Tested in WB, IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SIN3B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06424)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human SIN3B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06424 is reactive to Sin3b in Human
Applications
A06424 is guaranteed for IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
133066 MW
Background of Sin3b
Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3B to DNA. Also forms a complex with FOXK1 which represses transcription By similarity.
Ishikawa K., DNA Res. 5:169-176(1998).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Wysocka J., Genes Dev. 17:896-911(2003).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from A549 cells, using SIN3B antibody A06424.
Click image to see more details
Immunofluorescence analysis of HeLa cells, using SIN3B antibody A06424.
Protein Target Info & Infographic
Gene/Protein Information For Sin3b (Source: Uniprot.org, NCBI)
Gene Name
Sin3b
Full Name
Paired amphipathic helix protein Sin3b
Weight
133066 MW
Alternative Names
Histone deacetylase complex subunit Sin3b; KIAA0700paired amphipathic helix protein Sin3b; SIN3 homolog B, transcription regulator (yeast); SIN3 homolog B, transcriptional regulator (yeast); SIN3 homolog B, transcriptional regulator; Transcriptional corepressor Sin3b Sin3b|2810430C10Rik|transcriptional regulator, SIN3B (yeast)|paired amphipathic helix protein Sin3b|SIN3 homolog B, transcription regulator|histone deacetylase complex subunit Sin3b|transcriptional corepressor Sin3b|transcriptional regulator, SIN3 yeast homolog B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Sin3b, check out the Sin3b Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Sin3b: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SIN3B Antibody (A06424)
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No publications found for A06424
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Customer Q&As
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5 Customer Q&As for Anti-SIN3B Antibody
Question
Is a blocking peptide available for product anti-SIN3B antibody (A06424)?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
We do provide the blocking peptide for product anti-SIN3B antibody (A06424). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-09
Question
We are currently using anti-SIN3B antibody A06424 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
The anti-SIN3B antibody (A06424) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-20
Question
Will anti-SIN3B antibody A06424 work for WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-01-31
Answer
According to the expression profile of cervix carcinoma, SIN3B is highly expressed in cervix carcinoma. So, it is likely that anti-SIN3B antibody A06424 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2019-01-31
Question
I am interested in to test anti-SIN3B antibody A06424 on human cervix carcinoma for research purposes, then I may be interested in using anti-SIN3B antibody A06424 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-10-22
Answer
The products we sell, including anti-SIN3B antibody A06424, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-10-22
Question
Can you help my question with product A06424, anti-SIN3B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-05-24
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A06424 anti-SIN3B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-05-24