Anti-Thyroglobulin (Thyroidal Cell Marker) TG Monoclonal Antibody

Product Name

Anti-Thyroglobulin (Thyroidal Cell Marker) TG Monoclonal Antibody

View all Thyroglobulin Antibodies

SKU/Catalog Number

M00359-1

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Thyroglobulin (Thyroidal Cell Marker) TG Monoclonal Antibody (Catalog # M00359-1). Tested in ELISA, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Thyroglobulin (Thyroidal Cell Marker) TG Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00359-1)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: 6E1

Isotype

IgG1, kappa

Immunogen

Human thyroid follicular cells

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00359-1 is reactive to TG in Human

Reconstitution

Reconstitute with 1ml water (15 minutes at room temperature).

Calculated molecular weight

304790 MW

Background of Thyroglobulin

Thyroglobulin is a 660kDa dimeric pre-protein with mutiple glycosylation sites. It is produced by and processed within the thyroid gland to produce the hormone thyroxine and triiodothyronine. Â Prior to forming dimers, thyroglobulin monomers undergo conformational maturation in the endoplasmic reticulation. Â The vast majority of follicular carcinomas of the thyroid will give positive immunoreactivity for anti-thyroglobulin even though sometimes only focally. Poorly differentiated carcinomas of the thyroid are frequently anti-thyroglobulin negative. Adenocarcinomas of other-than-thyroid origin do not react with this antibody. This antibody is useful in identification of thyroid carcinoma of the papillary and follicular types. Presence of thyroglobulin in metastatic lesions establishes the thyroid origin of tumor. Anti-thyroglobulin, combined with anti-calcitonin, can identify medullary carcinomas of the thyroid. Furthermore, anti-thyroglobulin, combined with anti-TTF1, can be a reliable marker to differentiate between primary thyroid and lung neoplasms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00359-1 is guaranteed for ELISA, IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
Immunohistology (Formalin-fixed) (0.1-0.2ug/ml
30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For TG (Source: Uniprot.org, NCBI)

Alternative Names

Thyroglobulin;Tg;TG; TG AITD3N, TG thyroglobulin thyroglobulin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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