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Product Info Summary
| SKU: | A02026-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-Insig1 Antibody Picoband®
SKU/Catalog Number
A02026-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Insig1 Antibody Picoband® catalog # A02026-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Insig1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02026-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of rat Insig1, which shares 71.4% and 92.9% amino acid (aa) sequence identity with human and mouse Insig1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02026-1 is reactive to Insig1 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
30 kDa
Calculated molecular weight
37492 MW
Background of INSIG-1
Insulin induced gene 1, also known as INSIG1, is a protein which in humans is encoded by the INSIG1 gene. This gene encodes an endoplasmic reticulum membrane protein that regulates cholesterol metabolism, lipogenesis, and glucose homeostasis. The encoded protein has six transmembrane helices which contain an effector protein binding site. It binds the sterol-sensing domains of sterol regulatory element-binding protein (SREBP) cleavage-activating protein (SCAP) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase), and is essential for the sterol-mediated trafficking of these two proteins. It promotes the endoplasmic reticulum retention of SCAP and the ubiquitin-mediated degradation of HMG-CoA reductase. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02026-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Mouse
Positive Control
WB: rat liver tissue, rat lung tissue
FCM: HEPA1-6 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Insig1 using anti-Insig1 antibody (A02026-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat lung tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Insig1 antigen affinity purified polyclonal antibody (Catalog # A02026-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Insig1 at approximately 30KD. The expected band size for Insig1 is at 30KD.
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Figure 2. Flow Cytometry analysis of HEPA1-6 cells using anti-Insig1 antibody (A02026-1).
Overlay histogram showing HEPA1-6 cells stained with A02026-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Insig1 Antibody (A02026-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For Insig1 (Source: Uniprot.org, NCBI)
Gene Name
Insig1
Full Name
Insulin-induced gene 1 protein
Weight
37492 MW
Superfamily
INSIG family
Alternative Names
Tripartite motif-containing protein 6; RING finger protein 89; RING-type E3 ubiquitin transferase TRIM6; TRIM6; RNF89 INSIG1 CL6 insulin induced gene 1 insulin-induced gene 1 protein|INSIG-1 membrane protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Insig1, check out the Insig1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Insig1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Insig1 Antibody Picoband® (A02026-1)
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