Boster Bio Anti-SREBF2 Antibody (Catalog # A01678). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
SREBF2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-SREBF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01678)
Host
Rabbit
Contents
SREBF2 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SREBF2 antibody was raised against a 15 amino acid peptide near the center of human SREBF2. The immunogen is located within amino acids 440 - 490 of SREBF2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
SREBF2 antibody is human and mouse reactive. At least three isoforms of SREBF2 are known to exist. SREBF2 antibody is predicted not to cross-react with SREBF1.
Reactive Species
A01678 is reactive to SREBF2 in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
123688 MW
Background of SREBP2
The sterol regulatory element binding transcription factor 2 (SREBF2) is a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis (1,2). The related protein SREBF1 also binds SRE1 and activates transcription in an additive fashion to SREBF2 (2). SREBF2 has been more closely associated with cholesterol synthesis and accumulation, while the SREBF1 proteins are important in the regulation of genes involved in lipid metabolism (3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SREBF2 antibody can be used for detection of SREBF2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SREBF2 in PC-3 cell lysate with SREBF1 antibody at 1 μg/ml.
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Immunohistochemistry of SREBF2 in human spleen tissue with SREBF2 antibody at 5 μg/mL.
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Immunofluorescence of SREBF2 in human spleen tissue with SREBF2 antibody at 20 μg/mL.
Gene/Protein Information For SREBF2 (Source: Uniprot.org, NCBI)
Gene Name
SREBF2
Full Name
Sterol regulatory element-binding protein 2
Weight
123688 MW
Superfamily
SREBP family
Alternative Names
SREBP2, bHLHd2, SREBP-2, BHLHD2, SREBP2, Sterol regulatory element-binding protein 2, Class D basic helix-loop-helix protein 2 SREBF2 SREBP-2, SREBP2, bHLHd2 sterol regulatory element binding transcription factor 2 sterol regulatory element-binding protein 2|class D basic helix-loop-helix protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on SREBF2, check out the SREBF2 Infographic
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In this infographic, you will see the following information for SREBF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$449.00
Troubleshooting
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