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Product Info Summary
| SKU: | M00759 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TGF beta Receptor II TGFBR2 Rabbit Monoclonal Antibody
View all TGF-beta RII Antibodies
SKU/Catalog Number
M00759
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-TGF beta Receptor II TGFBR2 Rabbit Monoclonal Antibody catalog # M00759. Tested in WB application. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TGF beta Receptor II TGFBR2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00759)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ABFE-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human TGF beta Receptor II
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00759 is reactive to TGFBR2 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
20 kDa
Calculated molecular weight
64568 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00759 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of TGF beta Receptor II expression in A549 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For TGFBR2 (Source: Uniprot.org, NCBI)
Gene Name
TGFBR2
Full Name
TGF-beta receptor type-2
Weight
64568 MW
Superfamily
protein kinase superfamily
Alternative Names
TGF-beta receptor type-2;TGFR-2;2.7.11.30;TGF-beta type II receptor;Transforming growth factor-beta receptor type II;TGF-beta receptor type II;TbetaR-II;TGFBR2; TGFBR2 AAT3, FAA3, LDS1B, LDS2, LDS2B, MFS2, RIIC, TAAD2, TBR-ii, TBRII, TGFR-2, TGFbeta-RII transforming growth factor beta receptor 2 TGF-beta receptor type-2|TGF-beta receptor type IIB|TGF-beta type II receptor|tbetaR-II|transforming growth factor beta receptor II|transforming growth factor beta receptor type IIC|transforming growth factor, beta receptor II (70/80kDa)|transforming growth factor, beta receptor II alpha|transforming growth factor, beta receptor II beta|transforming growth factor, beta receptor II delta|transforming growth factor, beta receptor II epsilon|transforming growth factor, beta receptor II gamma
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TGFBR2, check out the TGFBR2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TGFBR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TGF beta Receptor II TGFBR2 Rabbit Monoclonal Antibody (M00759)
Hello CJ!
M00759 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of Kuijie Granule on the Expression of TGF-?/Smads Signaling Pathway in Patients with Ulcerative Colitis
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-TGF beta Receptor II TGFBR2 Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-TGF beta Receptor II Rabbit Monoclonal antibody for WB for mouse cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
It shows on the product datasheet, M00759 anti-TGF beta Receptor II Rabbit Monoclonal antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-13
Question
Is a blocking peptide available for product anti-TGF beta Receptor II Rabbit Monoclonal antibody (M00759)?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
We do provide the blocking peptide for product anti-TGF beta Receptor II Rabbit Monoclonal antibody (M00759). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-28
Question
We are currently using anti-TGF beta Receptor II Rabbit Monoclonal antibody M00759 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
The anti-TGF beta Receptor II Rabbit Monoclonal antibody (M00759) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-21
Question
We want using your anti-TGF beta Receptor II Rabbit Monoclonal antibody for response to cholesterol studies. Has this antibody been tested with western blotting on a549 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
We appreciate your inquiry. This M00759 anti-TGF beta Receptor II Rabbit Monoclonal antibody is validated on a549 cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-01-09
Question
Is this M00759 anti-TGF beta Receptor II Rabbit Monoclonal antibody reactive to the isotypes of TGFBR2?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
The immunogen of M00759 anti-TGF beta Receptor II Rabbit Monoclonal antibody is A synthesized peptide derived from human TGF beta Receptor II. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-12
Question
I have a question about product M00759, anti-TGF beta Receptor II Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00759 anti-TGF beta Receptor II Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-19
Question
See below the WB image, lot number and protocol we used for cervix carcinoma using anti-TGF beta Receptor II Rabbit Monoclonal antibody M00759. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-16
Question
We have been able to see staining in mouse placenta. Are there any suggestions? Is anti-TGF beta Receptor II Rabbit Monoclonal antibody supposed to stain placenta positively?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
From what I have seen in literature placenta does express TGFBR2. From what I have seen in Uniprot.org, TGFBR2 is expressed in metanephric glomerulus, liver, glial cell, placenta, cervix carcinoma, among other tissues. Regarding which tissues have TGFBR2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976, 23186163
Glial cell, Pubmed ID: 7959019
Liver, Pubmed ID: 1310899, 8973329, 24275569
Placenta, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-08-26
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-TGF beta Receptor II Rabbit Monoclonal antibody M00759. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-23
Question
Would anti-TGF beta Receptor II Rabbit Monoclonal antibody M00759 work for WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-07-17
Answer
According to the expression profile of cervix carcinoma, TGFBR2 is highly expressed in cervix carcinoma. So, it is likely that anti-TGF beta Receptor II Rabbit Monoclonal antibody M00759 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2019-07-17
Question
Do you have a BSA free version of anti-TGF beta Receptor II Rabbit Monoclonal antibody M00759 available?
Verified Customer
Verified customer
Asked: 2019-03-06
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TGF beta Receptor II Rabbit Monoclonal antibody M00759 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-03-06
Question
Will M00759 anti-TGF beta Receptor II Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Williams
Verified customer
Asked: 2017-07-31
Answer
It shows on the product datasheet, M00759 anti-TGF beta Receptor II Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-07-31
Question
I would like to test anti-TGF beta Receptor II Rabbit Monoclonal antibody M00759 on mouse cervix carcinoma for research purposes, then I may be interested in using anti-TGF beta Receptor II Rabbit Monoclonal antibody M00759 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-07-04
Answer
The products we sell, including anti-TGF beta Receptor II Rabbit Monoclonal antibody M00759, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-07-04
Question
My boss were well pleased with the WB result of your anti-TGF beta Receptor II Rabbit Monoclonal antibody. However we have been able to see positive staining in placenta cell membrane using this antibody. Is that expected? Could you tell me where is TGFBR2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-05-31
Answer
Based on literature, placenta does express TGFBR2. Generally TGFBR2 expresses in cell membrane. Regarding which tissues have TGFBR2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976, 23186163
Glial cell, Pubmed ID: 7959019
Liver, Pubmed ID: 1310899, 8973329, 24275569
Placenta, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-05-31
Question
I see that the anti-TGF beta Receptor II Rabbit Monoclonal antibody M00759 works with WB, what is the protocol used to produce the result images on the product page?
N. Jones
Verified customer
Asked: 2013-04-10
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-04-10
